| 4猪种Nramp1基因第6内含子多态性研究 |
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| 引用本文： | 赵生国,赵海、,潘虹,周艳红,滚双宝.4猪种Nramp1基因第6内含子多态性研究[J].中兽医医药杂志,2012,31(5):34-37. |
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| 作者姓名： | 赵生国 赵海、 潘虹 周艳红 滚双宝 |
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| 作者单位： | 甘肃农业大学动物科学技术学院,甘肃兰州,730070 |
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| 基金项目： | 国家自然科学基金青年基金项目（31101682）; 甘肃农业大学动物科学技术学院青年教师科研专项基金 |
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| 摘 要： | 天然抗性巨噬蛋白(Nramp)基因是与人、鼠的一些病原微生物的易感性和抗性有关的重要候选基因。为了研究猪Nramp1基因的多态性,利用PCR-RFLP技术检测了杜洛克、大白猪、长白猪和合作猪共270头个体Nramp1基因第6内含子NdeⅠ酶切位点多态性。结果表明,4个猪种群共检测到3种基因型(AA、AB和BB),其中AB基因型为杜洛克、大白猪和长白猪的优势基因型;AA基因型为合作猪的优势基因型。经卡方适合性检测,杜洛克、合作猪和长白猪处于Hardy-Weinberg平衡状态(P>0.05),大白猪处于Hardy-Weinberg不平衡状态。多态信息含量分析显示,Nramp1基因的第6内含子NdeⅠ酶切位点在各猪种表现出中度多态性。
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| 关 键 词： | Nramp1基因 第6内含子 PCR-RFLP 多态性 |
Study on the polymorphism in Nrampl gene intron 6 of four pig populations |
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| ZHAO Sheng.guo,ZHAO Hai,PAN Hong,ZHOU Yan-hong,GUN Shuang-bao.Study on the polymorphism in Nrampl gene intron 6 of four pig populations[J].Journal of Traditional Chinese Veterinary Medicine,2012,31(5):34-37. |
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| Authors: | ZHAO Shengguo ZHAO Hai PAN Hong ZHOU Yan-hong GUN Shuang-bao |
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| Institution: | (College of Ammal Science and I ethnology, Gansu Agricultural Umverslty, lnzhou Gansu 730070, China) |
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| Abstract: | Nramp1 gene（natural resistance associated macrophage protein） is the important candidate genes related with susceptibility and resistance of pathogenic microorganism in human and rats.In this study,the total of 270 pigs distributed in Duroc pigs,Large White pigs,Landrace pigs and Hezuo pigs were tested by PCR-RFLP for the polymorphism of Nramp1 gene intron 6.Three genotypes（AA,AB and BB） were detected in these four populations,AB was domination genotype in Duroc pigs,Large White pigs and Landrace pig,while AA was domination genotype in Hezuo pigs.Chi-square test indicated that three populations of Hezuo pigs,Duroc pigs and Landrace pigs were all in Hardy Weinberg balance state（P〉0.05） except Large White pigs.Gene frequencies and genotype frequencies analysis showed that the restriction sites of Nramp1 gene intron 6 in all pigs were moderately polymorphic. |
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| Keywords: | Nrampl gene intron 6 PCR-RFLP polymorphism |
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