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| 抗猪附红细胞体单克隆抗体的制备 | |
| 引用本文： | 张浩,吴晓东,张守富,单虎,吴延功,王志亮,刘佩兰,朱绍辉.抗猪附红细胞体单克隆抗体的制备[J].中国预防兽医学报,2007,29(10):814-816. |
| 作者姓名： | 张浩 吴晓东 张守富 单虎 吴延功 王志亮 刘佩兰 朱绍辉 |
| 作者单位： | 1. 莱阳农学院,动物科技学院,山东,青岛,266109;中国动物卫生与流行病学中心,山东,青岛,266032 2. 中国动物卫生与流行病学中心,山东,青岛,266032 3. 青岛中仁药业有限公司,山东,青岛,266329 4. 莱阳农学院,动物科技学院,山东,青岛,266109 |
| 摘 要： | 以纯化的猪附红细胞体免疫BALB/c小鼠,运用淋巴细胞杂交瘤技术进行细胞融合,并用间接ELISA方法进行筛选,经过间接ELISA方法、免疫印迹和间接免疫荧光试验进行鉴定,共获得5株分泌抗猪附红细胞体单克隆抗体的杂交瘤细胞,分别命名为1H1、1H2、3A5、5B1和7E11,其单抗亚类鉴定分别属于IgG2b、IgG1、IgG2b、IgG2b和IgG2b。这5株McAb均能与猪附红细胞体全菌蛋白发生特异性反应,而不与猪肺炎支原体、猪链球菌、大肠杆菌和猪繁殖与呼吸综合征病毒发生反应,并且1H1、3A5和5B1能识别同一抗原位点,1H2和7E11识别另一抗原位点。 |
| 关 键 词： | 杂交瘤 猪附红细胞体 单克隆抗体 间接ELISA |
| 文章编号： | 1008-0589（2007）10-0814-03 |
| 修稿时间： | 2006-12-28 |
Development of monoclonal antibodies against Eperythrozoon suis |
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| ZHANG Hao,WU Xiao-dong,ZHANG Shou-fu,SHAN Hu,WU Yan-gong,WANG Zhi-liang,LIU Pei-lan,ZHU Shao-hui.Development of monoclonal antibodies against Eperythrozoon suis[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(10):814-816. | |
| Authors: | ZHANG Hao WU Xiao-dong ZHANG Shou-fu SHAN Hu WU Yan-gong WANG Zhi-liang LIU Pei-lan ZHU Shao-hui |
| Institution: | 1. College of Animal Science and Technology, Laiyang Agricultural College, Qingdao 266109, China; 2. China Animal Health and Epidemiology Center, Qingdao 266032, China; 3. Qingdao Zhong Ren Pharmaceutical Co., LTD, Qingdao 266329, China |
| Abstract: | Monoclonal antibodies(McAb) were prepared using spleen cells of BALB/c mice immunized with purified Epery-throzoon suis according to standard hybridoma protocols.Five hybridoma cell lines secreting McAbs against E.suis were obtained following screening by indirect enzyme-linked immunosorbent assay(ELISA) and indirect immunofluorescence assay.These five McAbs,designated as 1H1,1H2,3A5,5B1 and 7E11 respectively,were highly specific for E.suis and didn't react with Mycoplasma hyopneumoniae,Streptococcus suis,E.coli,PRRSV.McAbs 1H1,3A5 and 5B1 were shown against the same epitope while 1H2 and 7E11 against another. |
| Keywords: | hybridoma eperythrozoon suis monoclonal antibody indirect ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |