| 重组马传染性贫血病病毒核心蛋白抗原在 A GID 和ELISA中应用的评价 |
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| 引用本文： | 孔宪刚,陶伟英,刘永刚,张宝山,刘胜旺,卢景良.重组马传染性贫血病病毒核心蛋白抗原在 A GID 和ELISA中应用的评价[J].中国预防兽医学报,1999(4). |
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| 作者姓名： | 孔宪刚 陶伟英 刘永刚 张宝山 刘胜旺 卢景良 |
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| 摘 要： | 用昆虫杆状病毒表达系统获得的马传染性贫血病病毒（ Ｅ Ｉ Ａ Ｖ） 核心蛋白（ Ｇａｇ） 和 Ｐ２６ 蛋白， 作为免疫琼脂双扩散（ Ａ Ｇ Ｉ Ｄ） 和酶联免疫吸附试验（ Ｅ Ｌ Ｉ Ｓ Ａ） 抗原。对７６ 份已知马传贫非特异性血清进行检查， 同时与市售 Ａ Ｇ Ｉ Ｄ 和 Ｅ Ｌ Ｉ Ｓ Ａ 试剂盒作比较。证明， 用表达蛋白作抗原的 Ａ Ｇ Ｉ Ｄ 和 Ｅ Ｌ Ｉ Ｓ Ａ 检测结果均为阴性反应， 而用市售 Ａ Ｇ Ｉ Ｄ 试剂盒检查有５４ 份马血清出现非特异性反应， 市售 Ｅ Ｌ Ｉ Ｓ Ａ 试剂盒检查也出现了非特异性反应， Ｏ Ｄ 值比表达抗原 Ｅ Ｌ Ｉ Ｓ Ａ 高３５ 倍。初步证明在 Ａ Ｇ Ｉ Ｄ 和 Ｅ Ｌ Ｉ Ｓ Ａ 法中， 表达抗原优于常规马传贫病毒抗原。
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| 关 键 词： | 马传染性贫血病病毒 杆状病毒 核心蛋白 |
Evaluation of Equine Infectious Anemia Virus Core Proteins Produced in a Baculovirus Expression System in Agar Gel Immunodiffusion Test and Enzyme linked Immunosorbent Assay |
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| KONG Xiangang,TAOWeiying,LIU Yonggang,ZHANG Baoshan,LIU Shengwang,LU Jingliang.Evaluation of Equine Infectious Anemia Virus Core Proteins Produced in a Baculovirus Expression System in Agar Gel Immunodiffusion Test and Enzyme linked Immunosorbent Assay[J].Chinese Journal of Preventive Veterinary Medicine,1999(4). |
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| Authors: | KONG Xiangang TAOWeiying LIU Yonggang ZHANG Baoshan LIU Shengwang LU Jingliang |
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| Abstract: | Equine infectious anemia virus(EIAV)core proteins(Gag and p26)obtained from a baculovirus expression system were used in agar gel immunodiffusion(AGID)and enzyme linked immunosorbent assay(ELISA)antigens to test seventy six horse sera.These sera showed false positive reaction in AGID test using Nisseiken antigen.However,none of them showed false positive reaction with the expressed antigen.The 76 horse sera were also tested by ELISA.The sera gave a high background also in ELISA using Nisseiken antigen. |
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| Keywords: | EIAV Baculovirus Core protein |
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