| 两株高致病性PRRSV ORF6和ORF7基因克隆及序列分析 |
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| 引用本文： | 时建立,李俊,徐绍建,赵鸿雁,张秀美,王金宝.两株高致病性PRRSV ORF6和ORF7基因克隆及序列分析[J].中国畜牧兽医,2008,35(1):39-43. |
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| 作者姓名： | 时建立 李俊 徐绍建 赵鸿雁 张秀美 王金宝 |
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| 作者单位： | 1青岛农业大学，青岛 266109； 2山东省农科院畜牧兽医研究所，济南 250100 |
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| 基金项目： | 山东省自然基金重大项目(Z2007D06) |
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| 摘 要： | 应用RT PCR方法从实验室分离的两株高致病性PRRSV SX、ZQ株中扩增出ORF6和ORF7，将其分别克隆、测序。用DNAStar 软件分析所测序列,并与VR 2332株、LV株、周边国家及国内分离株进行核苷酸和推导氨基酸同源性比较，并绘制系统进化树，结果ORF6、ORF7核苷酸与北美洲型的同源性为91.1％～100％，与欧洲型的同源性为66.2％～70.7％，推导氨基酸与北美洲型的同源性为91.2％～100％，与欧洲型的同源性为62.3％～82.3％。证明新分离到的PRRSV SX株、ZQ株仍属北美洲型。SX株ORF6、ORF7核苷酸与国内新分离到的高致病性PRRSV JXA1株同源性分别为99.8%、100%；ZQ株ORF6、ORF7核苷酸与国内新分离到的高致病性PRRSV JXA1株同源性分别为99.6%、99.7%。
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| 关 键 词： | 高致病性PRRSV ORF6 ORF7 克隆 序列分析 |
| 文章编号： | 1670-7236（2008）01-0039-05 |
| 收稿时间： | 2007-07-09 |
| 修稿时间： | 2007年7月9日 |
Clone and Sequence Analysis of Two Highly Pathogenic PRRSV ORF6 and ORF7 Genes |
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| SHI Jian-li,LI Jun,XU Shao-jian,ZHAO Hong-yan,ZHANG Xiu-mei,WANG Jin-bao.Clone and Sequence Analysis of Two Highly Pathogenic PRRSV ORF6 and ORF7 Genes[J].China Animal Husbandry & Veterinary Medicine,2008,35(1):39-43. |
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| Authors: | SHI Jian-li LI Jun XU Shao-jian ZHAO Hong-yan ZHANG Xiu-mei WANG Jin-bao |
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| Institution: | 1. Qingdao Agricultural University, Qingdao 266109，China;2.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences, Jinan 250100,China |
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| Abstract: | The ORF6 and ORF7 genes of two highly pathogenic PRRSV SX,ZQ,derived from Shandong,were amplified by RT-PCR.The products were sequenced after cloning into pMD18-T vector respectively .The sequences were analysed using DNAStar and compared with those of VR-2332,LV and other strains isolated from China and circumjacent Country.The homologous of ORF6,ORF7 Nuclear acid was 91.1％～100％ with North American(VR-2332) and 66.2％～70.7％ with European(LV) viruses, The homologous of ORF6,ORF7 deduced amino acid was 91.2％～100％ with North American(VR-2332) and 62.3％～82.3％ with European(LV) viruses,indicating that the two new shandong isolates were related to the North American PRRSV genotype. The ORF6,ORF7 Nuclear acid of SX shared 99.8%,100% identity with highly pathogenic PRRSV JXA1,while The ORF6,ORF7 Nuclear acid of ZQ shared 99.6%,99.7% identity with highly pathogenic PRRSV JXA1. |
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| Keywords: | highly pathogenic PRRSV ORF6 ORF7 clone sequence analysis |
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