| 具有HI活性的抗H5亚型流感病毒特异性单克隆抗体的研制 |
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| 引用本文： | 张立春,邓国华,刘振勇,田国彬,于康震,陈化兰.具有HI活性的抗H5亚型流感病毒特异性单克隆抗体的研制[J].中国预防兽医学报,2005,27(5):329-332. |
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| 作者姓名： | 张立春 邓国华 刘振勇 田国彬 于康震 陈化兰 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,农业部动物流感重点开放实验室/兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 以H5N1亚型禽流感病毒分离株A/Duck/Zhejiang/11/00(H5N1)(DZJ/1100)作为免疫原,浓缩纯化后免疫BALB/c小鼠,三次免疫后,取免疫小鼠脾细胞与SP2/O细胞融合,融合阳性细胞用血凝抑制(HI)方法进行检测,阳性细胞株经三代克隆纯化后,获得三株能稳定分泌抗血凝素特异性HI单克隆抗体的杂交瘤细胞株,分别命名为DD7、FG10、CG12.三株杂交瘤细胞株产生的小鼠腹水和细胞培养液上清的HI滴度分别是213、215、211和27、8、23.与其他具有血凝活性的禽类病毒以及其他14个HA亚型的禽流感病毒的交叉HI试验表明:这三株单抗具有良好的禽流感病毒亚型特异性;与其他H5亚型流感病毒分离株的HI试验和中和试验证实这三株单抗具有良好的交叉性(分别为7/8、8/8、8/8)和中和活性(6/8、8/8、8/8).该亚型特异性单克隆抗体的研制成功为H5亚型禽流感病毒的疫情病原学快速诊断提供了坚实的物质保障.
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| 关 键 词： | 禽流感 血凝抑制试验 单克隆抗体 |
| 文章编号： | 1008-0589(2005)05-0329-04 |
| 收稿时间： | 2004-04-08 |
| 修稿时间： | 2004年4月8日 |
Development of monoclonal antibodies specific to H5 subtype avian influenza virus with HI characteristic |
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| ZHANG Li-chun,DENG Guo-hua,LIU Zhen-yong,TIAN Guo-bin,YU Kang-zhen,CHEN Hua-lan.Development of monoclonal antibodies specific to H5 subtype avian influenza virus with HI characteristic[J].Chinese Journal of Preventive Veterinary Medicine,2005,27(5):329-332. |
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| Authors: | ZHANG Li-chun DENG Guo-hua LIU Zhen-yong TIAN Guo-bin YU Kang-zhen CHEN Hua-lan |
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| Abstract: | Three monoclonal antibodies specific to H5 subtype avian influenza virus with HI characteristic were developed by fusing between sp2/0 myeloma cells and spleen cells of Balb/c mice immunized with the condensed and purified H5 influenza virus.Three of them were designed as DD7,FG10 and CB12,which HI titers of supernatant culture and the ascites were 2~(7),2~(8),2~(3) and 2~(13),2~(15),2~(11) respectively.In this study,these McAbs were specific to H5 subtype AIV-,and not reacted with AIV-H1-H4,H6-H14 subtype AIV and others avian viruses by hemagglutinin inhibition test(HI).The monoclonal antibodies have the favorable antigen capture and neutralization faculties to H5 AIV.These results suggested that the monoclonal antibodies would be useful tool in H5 subtype influenza virus surveillance. |
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| Keywords: | H5 subtype avian influenza HI mcAb |
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