| 副猪嗜血杆菌国内流行血清型对小鼠外周血淋巴细胞·细胞因子及免疫球蛋白水平的影响 |
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| 引用本文： | 陈贯超,廖成水.副猪嗜血杆菌国内流行血清型对小鼠外周血淋巴细胞·细胞因子及免疫球蛋白水平的影响[J].安徽农业科学,2016,44(16):164-166. |
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| 作者姓名： | 陈贯超 廖成水 |
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| 作者单位： | 汝州市动物卫生监督所,河南汝州,467500;河南科技大学，动物疫病与公共安全重点实验室/河南省动物疫病与公共安全院士工作站，河南洛阳471003 |
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| 基金项目： | 国家自然科学基金面上项目(31572489)，河南科技大学青年科学基金项目(2015QN033)。 |
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| 摘 要： | 目的]比较副猪嗜血杆菌国内流行血清型菌株感染后小鼠外周血淋巴细胞、血清细胞因子及免疫球蛋白水平的变化情况。方法]将72只小鼠分为4组,分别腹腔注射副猪嗜血杆菌血清型4、5和12菌株,检测3 d、7 d和15 d各组外周血T、B淋巴细胞及血清细胞因子和Ig水平。结果]副猪嗜血杆菌感染小鼠3 d,T、B淋巴细胞数量明显高于对照组,而随着感染时间的延长而逐渐下降。Ig G、IFN-γ和IL-10水平随感染时间的延长而逐渐增高,而Ig A、Ig M和Ig E水平逐渐降低。感染3 d,Th1/Th2比值稍有下降,而Th1/Th2比值随着感染时间的延长而逐渐升高,明显高于对照组。不同血清型感染在各时间点之间T、B淋巴细胞、Ig A、Ig M、Ig E、IFN-γ、IL-10和Th1/Th2差异均无统计学意义,而血清型4和5感染组小鼠血清Ig G均明显高于血清型12组。结论]除Ig G以外,副猪嗜血杆菌国内流行血清型4、5和12菌株感染小鼠后外周血淋巴细胞、血清细胞因子及Ig水平无明显差异。
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| 关 键 词： | 副猪嗜血杆菌 血清型 淋巴细胞 细胞因子 免疫球蛋白 |
Level of Peripheral Blood Lymphocytes,Cytokines and Immunoglobulins in Mice Infected with the Epidemic Serotypes Haemophilus parasuis in China |
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| CHEN Guan-chao,LIAO Cheng-shui.Level of Peripheral Blood Lymphocytes,Cytokines and Immunoglobulins in Mice Infected with the Epidemic Serotypes Haemophilus parasuis in China[J].Journal of Anhui Agricultural Sciences,2016,44(16):164-166. |
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| Authors: | CHEN Guan-chao LIAO Cheng-shui |
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| Abstract: | Objective] To comparatively study on level of peripheral blood lymphocytes, cytokines, and immunoglobulins ( Ig) in mice infec-ted with the epidemic serotypes Haemophilus parasuis in China. Method] 72 mice were subdivided into four groups, group serotypes 4, 5, 12, and PBS (control group), and were injected intraperitoneally with Haemophilus parasuis serotypes 4, 5, 12, and PBS. T lymphocytes and B lymphocytes, cytokines, and Ig were detected. Result] The number of T lymphocytes and B lymphocytes in mice on 3 days after infection with Haemophilus parasuis, and gradually decreased as increasing time. But gradually increased in the level of IgG, IFN-γ, and IL-10, and gradually decreased in the level of IgA, IgM, and IgE. The Th1/Th2 level was gradually increased as increasing time, and significantly higher than the control group. However, there were no significant differences between different serotypes Haemophilus parasuis in the level of T lym-phocytes and B lymphocytes, IgA, IgM, IgE, IFN-γ, IL-10, and Th1/Th2, but the level of IgG in group serotypes 4 and 5 were significantly higher than group serotypes 12. Conclusion] There were no significant differences in the level of peripheral blood lymphocytes, cytokines, and Ig except IgG in mice infected with Haemophilus parasuis serotypes 4, 5, 12 in China. |
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| Keywords: | Haemophilus parasuis Serotypes Lymphocytes Cytokines Immunoglobulins |
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