| 抗κ-酪蛋白单克隆抗体的制备及其特性鉴定 |
| |
| 引用本文： | 张莹,周玉,李岩松,等.抗κ-酪蛋白单克隆抗体的制备及其特性鉴定[J].中国畜牧兽医,2014,41(3):24-27. |
| |
| 作者姓名： | 张莹 周玉 李岩松 等 |
| |
| 摘 要： |
|
| 关 键 词： | &kappa -酪蛋白 单克隆抗体 特性鉴定 |
| 收稿时间： | 2013-09-26 |
Preparation and Characteristics Identification of Anti-kappa Casein Monoclonal Antibody |
| |
| ZHANG Ying,ZHOU Yu,LI Yan-song,MENG Xing-yu,CHU Xiao-hong,LU Shi-ying,REN Hong-lin,HU Pan,LIU Zeng-shan,WANG Nan-nan,LI Dan.Preparation and Characteristics Identification of Anti-kappa Casein Monoclonal Antibody[J].China Animal Husbandry & Veterinary Medicine,2014,41(3):24-27. |
| |
| Authors: | ZHANG Ying ZHOU Yu LI Yan-song MENG Xing-yu CHU Xiao-hong LU Shi-ying REN Hong-lin HU Pan LIU Zeng-shan WANG Nan-nan LI Dan |
| |
| Institution: | (Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China) |
| |
| Abstract: | To obtain the anti-kappa casein monoclonal antibody and complete the identification of the antibody characteristics. The BALB/c mice were immunized with kappa casein using foot-pad immunization. Popliteal lymph node cells from the immunized mice were fused with SP2/0 myeloma cells in the presence of PEG. Three hybridoma strains (1C4, 3G3, 3E6) which secreted the antibody specific for kappa casein were obtained．The sub-class of the antibodies were IgG1. The ascites were purified by Protein G affinity layer absorption column. The antigenic epitope of 3G3 and 3E6 were different and it was close between 1C4 and 3E6.The titer of purified ascites(1C4) was 1.28×106 and the affinity constant was 2.89×108 mol/L. A anti-kappa casein monoclonal antibody with good affinity had been achieved，which provided foundations for the rapid detection of casein in bovine milk samples. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
