| 马立克氏病毒单克隆抗体杂交瘤细胞株的建立与鉴定 |
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| 引用本文： | 房德兴,刘作臣,殷震,夏咸柱,刘.马立克氏病毒单克隆抗体杂交瘤细胞株的建立与鉴定[J].中国兽医学报,1989(3). |
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| 作者姓名： | 房德兴 刘作臣 殷震 夏咸柱 刘 |
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| 作者单位： | 兽医大学军事兽医研究所病毒研究室
(房德兴，刘作臣，殷震，夏咸柱，刘(王子))，兽医大学军事兽医研究所病毒研究室(马从林) |
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| 摘 要： | 以马立克氏病毒814株(MDV-814)作为免疫用抗原,建立了2个MDV特异性单克隆抗体(McAb)杂交瘤细胞株—4A6、3D7。鉴定结果表明;两株细胞所分泌的McAb均为IgM类免疫球蛋白,具有MDVⅠ型病毒特异性,无中和反应特性和沉淀反应特性。利用两种McAb对国内标准强毒MDV-京1株进行鉴定,肯定其为MDV-Ⅰ型毒株。
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| 关 键 词： | 马立克氏病毒 单克隆抗体 |
Establishment and Characterization of Hybridoma Strains Secreting Monoclonal Antibody against Mareks Disease Virus |
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| Fang Dexing et al.Establishment and Characterization of Hybridoma Strains Secreting Monoclonal Antibody against Mareks Disease Virus[J].Chinese Journal of Veterinary Science,1989(3). |
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| Authors: | Fang Dexing |
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| Institution: | Fang Dexing et al. The Military Veterinary Institute,Veterinary College of PLA |
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| Abstract: | Two hybridoma cell strains of Marek's disease virus(MDV) specific McAb, 4A6 and 3D7, were established by MDV-814252 strain as immunc antigen. The identifying results showed that boththe McAbs belong to IgM class and are serotype I MDV specificantibodies. They have no virus-neutralization and precipitationabilities. MDV-BJ1 strain reacted specifically with both the serotypeI MDV McAbs in ELISA, thus it is futher confirmed that thischinese virulent MDV strain is serotype I MDV. |
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| Keywords: | Marek's disease virus monoclonal antibody |
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