| 观音土曲乳酸菌的分离与分子鉴定 |
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| 引用本文： | 李睿,沈汪洋,翟平平,陈道玉.观音土曲乳酸菌的分离与分子鉴定[J].安徽农业科学,2011,39(4):1891-1892,1894. |
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| 作者姓名： | 李睿 沈汪洋 翟平平 陈道玉 |
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| 作者单位： | 武汉工业学院生物与制药工程学院,湖北武汉,430023;武汉工业学院食品科学与工程学院,湖北武汉,430023;湖北劲牌有限公司,湖北大冶,435100 |
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| 基金项目： | 湖北省自然科学基金重点项目 |
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| 摘 要： | 目的]指导白酒生产,提高白酒质量。方法]采用平板分离法从观音土曲中分离到7株乳酸菌（B1~B7）,测定其发酵产乳酸的量。选取乳酸产量最高的1株菌B7,提取其DNA并进行16S rDNA的PCR扩增、克隆、测序。从GenBank中选取与B7同源性最高的典型菌株,下载其16S rDNA序列,采用邻位相连法构建进化树。结果]B1~B7的产乳酸量分别为：24.7、20.7、30.7、15.2、9.5、21.8、35.7mg/100m l;对B7的基因组DNA进行16S rDNA的PCR扩增,并对纯化后的扩增产物进行TA克隆,阳性克隆经琼脂糖凝胶电泳后在3000和1 600 bp处出现电泳条带,说明B7的16S rDNA PCR扩增产物已克隆到测序载体上。同源性分析结果表明,B7为乳杆菌属的短乳杆菌。结论]该研究从观音土曲中分离到1株高产乳酸菌——短乳杆菌。
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| 关 键 词： | 观音土曲 16S rDNA 乳酸菌 分离 鉴定 |
Isolation and Molecular Identification of Lactobacillus from White Clay Distillers Yeast |
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| LI Rui et al.Isolation and Molecular Identification of Lactobacillus from White Clay Distillers Yeast[J].Journal of Anhui Agricultural Sciences,2011,39(4):1891-1892,1894. |
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| Authors: | LI Rui |
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| Institution: | LI Rui et al(College of Biology and Pharmaceutical Engineering,Wuhan University of Industry,Wuhan,Hubei 430023) |
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| Abstract: | Objective]The purpose of this study was to direct the production of distillate spirit and enhance its quality.Method] Seven strains of lactobacilli(B1~B7) were isolated from white clay distillers yeast by plate isolation method and their lactic acid yields in fermentation was determined.The strain B7 with highest yield of lactic acid was selected for extracting DNA and performing PCR amplification,clone and sequencing on its 16S rDNA.The typical strains,which had highest homology with B7,were screened out... |
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| Keywords: | 16S rDNA |
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