Distribution of three GnRHs in the brain and pituitary of the wild Japanese flounder Paralichthys olivaceus |
| |
| Authors: | Ky Xuan Pham Masafumi Amano Noriko Amiya Yutaka Kurita Kunio Yamamori |
| |
| Institution: | School of Fisheries Sciences, Kitasato University, Ofunato, Iwate 022-0101 and;Tohoku National Fisheries Research Institute, Shiogama, Miyagi 985-0001, Japan |
| |
| Abstract: | ABSTRACT: Wild adult maturing and immature female Japanese flounder Paralichthys olivaceus were collected in June 2004 and January 2005, respectively, to clarify a possible role of gonadotropin-releasing hormones (GnRHs) in reproduction. Levels of salmon GnRH (sGnRH), chicken GnRH-II (cGnRH-II) and sea bream GnRH (sbGnRH) in the brain and pituitary were examined by time-resolved fluoroimmunoassay. Three forms of GnRHs were detected in the discrete brain at various levels. In the pituitary of both maturing and immature fish, sbGnRH was abundant together with a pronounced amount of sGnRH, whereas cGnRH-II was almost below the detectable limit. In maturing fish, levels of sbGnRH were high in the telencephalon, hypothalamus and pituitary, while levels of sbGnRH of immature fish were very low in these regions. These results indicate that sbGnRH is mainly responsible for gonadotropin secretion, and that sbGnRH in the anterior part of the brain is associated with gonadal maturation in the Japanese flounder. |
| |
| Keywords: | KEY WORDS: brain GnRH Japanese flounder pituitary time resolved fluoroimmunoassay TR-FIA |
| 本文献已被 SpringerLink 等数据库收录！ |
|
