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| IBV安徽分离株AH1-99M基因克隆及序列分析 | |
| 引用本文： | 何长生,魏建忠,刘胜旺.IBV安徽分离株AH1-99M基因克隆及序列分析[J].动物医学进展,2007,28(5):36-38. |
| 作者姓名： | 何长生 魏建忠 刘胜旺 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨,150001;安徽农业大学动物科技学院,安徽合肥,230036;安徽省兽医工作站,安徽合肥,230022 2. 安徽农业大学动物科技学院,安徽合肥,230036 3. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨,150001 |
| 摘 要： | 利用RT-PCR技术成功扩增出IBV分离株AH1-99 M基因全长cDNA,并将其克隆到pMD18-T载体上,获得该分离株M基因的重组质粒.序列分析结果表明,该分离株M基因全长共678个核苷酸,编码225个氨基酸;同其他IBV的核苷酸序列的同源性为87.2%～92.5%,氨基酸的同源性为89.8%～95.1%;在IBV M基因核苷酸进化关系树中,AH1-99株M基因与H120、M41、Beaudette以及多数国内分离株亲源关系较近. |
| 关 键 词： | 鸡传染性支气管炎病毒 AH1-99株 M基因 遗传变异分析 |
| 文章编号： | 1007-5038（2007）05-0036-03 |
| 收稿时间： | 2007-02-26 |
| 修稿时间： | 2007年2月26日 |
Cloning and Analysis of M Gene of Infectious bronchitis virus Anhui Strain AH1-99 |
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| HE Chang-sheng,WEI Jian-zhong,LIU Sheng-wang.Cloning and Analysis of M Gene of Infectious bronchitis virus Anhui Strain AH1-99[J].Progress In Veterinary Medicine,2007,28(5):36-38. | |
| Authors: | HE Chang-sheng WEI Jian-zhong LIU Sheng-wang |
| Abstract: | The whole cDNA of M gene of IBV AH1-99 strain was amplified by RT-PCR, and then was cloned into pMD18-T vector. The recombinant plasmid was proved to be positive by enzyme analysis and plasmid PCR. The nucleotide sequence analysis showed that the M gene was composed of 678 nucleotides and coded 225 amino acid residues. Compared with other IBV reference strains, the homology of nucleo- tides was between 87.2%-92.5% and shared 89.8%-95. 1% homology of amino acids with others. The phylogenetic analysis showed that it was closely related to H120, M41, Beaudette, and most of domestic IBV isolated strains. |
| Keywords: | IBV AH1-99 strain M gene phylogenetic analysis |
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