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基于16S rDNA序列和RFLP分析的病鳗分离菌株鉴定
引用本文:郭松林,关瑞章,冯建军,杨求华.基于16S rDNA序列和RFLP分析的病鳗分离菌株鉴定[J].厦门水产学院学报,2012(2):81-88.
作者姓名:郭松林  关瑞章  冯建军  杨求华
作者单位:[1]集美大学水产学院,福建厦门361021 [2]教育部鳗鱼产业技术工程研究中心,福建厦门361021
基金项目:[基金项目】国家自然科学基金(31001136);农业部公益性行业科研专项(3-51);福建省教育厅项目(JA10191);福建省教育厅资助省属高校科研专项(JK2011029)
摘    要:结合16S rDNA基因序列和限制性片段长度多态性(RFLP)的分析方法,通过与GenBank库中已递交的细菌16S rDNA基因序列进行同源性比较,对分离自发病鳗鲡(欧洲鳗鲡,日本鳗鲡和美洲鳗鲡)的30株细菌进行初步鉴定和分类.结果表明,这些细菌可大致分为气单胞菌属(Aeromonas sp.)、假单胞菌属(Pseudomonas sp.)、邻单胞菌属(Plesiomonas sp.)、克雷伯氏菌属(Klebsiella sp.)、柠檬酸杆菌属(Citrobacter sp.)、不动杆菌属(Acinetobacter sp.)和肠杆菌属(Enterobacter sp.)等7个菌属.分析认为,部分分离菌株可能是引起鳗鲡病害的疑似致病性菌株.

关 键 词:鳗鲡  细菌  16S  rDNA  RFLP  鉴定

Identification of Bacteria Isolated from Diseased Eels Using 16S rDNA PCR / RFLP Analysis
Authors:GUO Song-lin  GUAN Rui-zhang FENG Jian-jun  YANG Qiu-hua
Institution:1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Engineering Research Center of Eel Industrial Technology, Ministry of Education, Xiamen 361021, China)
Abstract:Using 16S rDNA gene sequences and restriction fragment length polymorphism (RFLP) a- nalysis, the study initially identified 30 strains of bacteria isolated from European eel (Anguilla anguilla) , Japanese eel (Anguilla japonica) and American eel (Anguilla rostrata) according to the homology compari- son with bacterial 16S rDNA gene sequence which had been submitted into the C, eneBank database. The re- sults showed that the 30 bacteria could be broadly divided into 7 genera, including Aeromonas sp. , Pseudo- monas sp. , P/es/omonas sp. , Klebsh:lla sp. , Citrobacter sp. , Acinetobacter sp. and Enterobacter sp. Re- sults of the study indicated some of the isolates could be suspected as pathogenic strains which caused diseases to eels.
Keywords:eel  bacteria  16S rDNA  RFLP  identification
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