| 广西陆川猪肺炎支原体P46基因克隆及序列分析 |
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| 引用本文： | 李莹莹,赵武,李斌,等.广西陆川猪肺炎支原体P46基因克隆及序列分析[J].中国畜牧兽医,2014,41(2):1-5. |
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| 作者姓名： | 李莹莹 赵武 李斌 等 |
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| 摘 要： |
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| 关 键 词： | 广西陆川猪 猪肺炎支原体 P46基因 克隆 序列分析 |
Cloning and Sequence Analysis of P46 Gene of Mycoplasma hyopneumoniae in Guangxi Luchuan Pig |
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| LI Ying-ying,ZHAO Wu,LI Bin,QIN Yi-bin,LU Bing-xia,LIANG Jia-xing,HE Ying,ZHOU Ying-ning,LU Qin-zhang.Cloning and Sequence Analysis of P46 Gene of Mycoplasma hyopneumoniae in Guangxi Luchuan Pig[J].China Animal Husbandry & Veterinary Medicine,2014,41(2):1-5. |
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| Authors: | LI Ying-ying ZHAO Wu LI Bin QIN Yi-bin LU Bing-xia LIANG Jia-xing HE Ying ZHOU Ying-ning LU Qin-zhang |
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| Institution: | (1.Guangxi Key Laboratory of Animal Vaccines and New Technology,Guangxi Veterinary Research Institute，Nanning 530001,China;2.College of Animal Science and Technology,Guangxi University,Nanning 530005,China) |
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| Abstract: | In order to understand the sequence characteristics, basic structure and genetic variation of P46 gene which was the main immunogenic surface membrane protein gene of local prevalent Mycoplasma hyopneumoniae (Mhp) strains in Guangxi Luchuan pig.The Mhp P46 gene in positive diseased swine samples which were collected from the purebred Luchuan pig farms in Guangxi from 2011 to 2013 was amplified using PCR,and then cloned into pMD18-T vector and transformed into E.coli DH5α.We chose the positive clones and sequenced.We amplified P46 genes of four positive strains (GXLC1,GXLC2,GXLC3 and GXLC4).Use the DNAStar software to analyse the cloned sequence.The results showed that P46 gene sequence was 1104 bp coding 368 amino acids.The sequence included three Trps coded by TGA codons which weren’t termination codons and one Arg coded by CGG codons which weren’t nonsense code.The homologies of nucleotide sequences of 4 strains were 98.4% to 99.4%,and the homologies of deduced amino acid sequences were 98.6% to 99.5% with these sequences of standard J strain,232 strain,7448 strain and 168 strain.The P46 genes of these strains had highly conservation because of the high-level homologies. |
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| Keywords: | Guangxi Luchuan pig Mycoplasma hyopneumoniae (Mhp) P46 gene clone sequence analysis |
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