| 益生菌互作对肉鸡生长性能、肠道消化吸收及糖转运蛋白GLUT2影响的研究 |
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| 引用本文： | 张利环,张若男,贾浩,马悦悦,朱芷葳,李慧锋,陈员玉.益生菌互作对肉鸡生长性能、肠道消化吸收及糖转运蛋白GLUT2影响的研究[J].畜牧兽医学报,2020,51(9):2165-2176. |
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| 作者姓名： | 张利环 张若男 贾浩 马悦悦 朱芷葳 李慧锋 陈员玉 |
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| 作者单位： | 山西农业大学 生命科学学院, 太谷 030801 |
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| 基金项目： | 山西省重点研发计划项目（201803D221023-2；201803D31062）；山西农业大学科技创新基金项目（2016ZZ07）；国家自然科学基金（31402156） |
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| 摘 要： | 旨在研究益生菌互作对肉鸡生长性能、肠道养分消化吸收及相关糖转运蛋白的影响。本研究中，200只1日龄雄性爱拔益加肉雏鸡被随机分为4组，每组5个重复，每个重复10只。将干酪乳杆菌、嗜酸乳杆菌和双歧杆菌分别按比例混合后于牛乳中发酵。对照组正常饮水（control组），益生菌Ⅰ组（混合比例为2：1：1）、Ⅱ组（混合比例为1：2：1）、Ⅲ组（混合比例为1：1：2）补充1%复合菌乳制品于饮用水中，各组皆饲喂基础日粮。整个试验饲养周期为42 d，每21 d为一个阶段。结果表明：1）与对照组相比，益生菌互作组均可显著提高肉鸡平均日增重（P<0.05），改善饲料利用率（P>0.05），在所有益生菌互作组中，益生菌Ⅰ组的肉鸡生长性能最好；2）益生菌互作显著刺激了空肠淀粉酶、脂肪酶、胰蛋白酶的特异性活性（P<0.05）；3）益生菌互作组均显著提高了小肠绒毛高度（P<0.05），降低了隐窝深度（P<0.05），上调了GLUT2 mRNA的表达（P<0.05），其中，以益生菌Ⅰ、Ⅲ组作用最佳。综上所述，益生菌互作能够增强消化酶活性、改善消化道结构、上调营养转运蛋白表达，提高养分的消化率及能量吸收有效性，从而提高肉鸡的生产性能；且当干酪乳杆菌、嗜酸乳杆菌和双歧杆菌的混合配比为2：1：1时，复合益生菌制剂对肉鸡的作用效果较佳。
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| 关 键 词： | 益生菌 肠道结构 消化吸收 GLUT2 |
| 收稿时间： | 2020-04-24 |
Effects of Probiotics Interaction on Growth Performance,Intestinal Digestion and Absorption,and Sugar Transporter GLUT2 in Broilers |
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| ZHANG Lihuan,ZHANG Ruonan,JIA Hao,MA Yueyue,ZHU Zhiwei,LI Huifeng,CHEN Yuanyu.Effects of Probiotics Interaction on Growth Performance,Intestinal Digestion and Absorption,and Sugar Transporter GLUT2 in Broilers[J].Acta Veterinaria et Zootechnica Sinica,2020,51(9):2165-2176. |
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| Authors: | ZHANG Lihuan ZHANG Ruonan JIA Hao MA Yueyue ZHU Zhiwei LI Huifeng CHEN Yuanyu |
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| Institution: | College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China |
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| Abstract: | The aim of this study was to investigate the effects of probiotic interaction on growth performance, intestinal nutrient digestion and absorption, and related sugar transporters in broiler. Two hundred 1-day-old male Arbor Acres broilers were selected, and then randomly divided into 4 groups with 5 replicates per group and 10 for each replicate. Lactobacillus casei, Lactobacillus acidophilus and Bifidobacterium lactis were mixed in proportion, and then fermented in milk. The chicks in the control group received normal drinking water, the chicks in probiotic group Ⅰ (mixed ratio of 2:1:1), group Ⅱ (mixed ratio of 1:2:1), and group III (mixed ratio of 1:1:2) received drinking water supplemented with 1% compound probiotics. All chickens were fed with normal diet for 42 days, every 21 days as a stage. The results showed that: 1) Compared to control group, the ADG(P<0.05) and feed conversion ratio (P>0.05) of broilers in all probiotic groups were improved. Furthermore, the production performance of broilers in probiotic group Ⅰ was higher than those of probiotic group Ⅱ and Ⅲ. 2) Compared to control group, specific activities of amylase, lipase, trypsin in jejunum of probiotic groups increased significantly (P<0.05). 3) In all probiotic groups, the intestinal villus height increased significantly (P<0.05), crypt depth decreased significantly (P<0.05), and the expression of glucose transporter 2 (GLUT2), particularly in probiotic group Ⅰ and Ⅲ, was significantly upregulated (P<0.05). In conclusion, probiotics interaction can enhance digestive enzyme activity, improve the structure of digestive tract, upregulate the expression of nutrient transporter, improve the nutrient digestibility and energy absorption efficiency, and further improve production performance of broiler. When the mixing ratio of L. casei, L. acidophilus and B. lactis was 2:1:1, the compound probiotics had a better effect on broilers. |
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| Keywords: | probiotics intestinal structure digestion and absorption GLUT2 |
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