| 小麦全蚀病生防芽孢杆菌的筛选与鉴定 |
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| 引用本文： | 吴贤兵,孙睿揆,吴毅歆,王志远,吴兴兴,毛自朝,何月秋.小麦全蚀病生防芽孢杆菌的筛选与鉴定[J].江西农业学报,2012(11):53-55,58. |
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| 作者姓名： | 吴贤兵 孙睿揆 吴毅歆 王志远 吴兴兴 毛自朝 何月秋 |
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| 作者单位： | 云南农业大学农学与生物技术学院;云南农业大学农业生物多样性与病虫害控制教育部重点实验室 |
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| 基金项目： | 科技部国际科技合作项目(2009DFA32360) |
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| 摘 要： | 利用菌饼对峙法从云南省的土样中分离出了234株对小麦全蚀病菌(Gaeumannomyces graminis var.tritici)有拮抗作用的细菌,其中Kc菌株使用浓度为3.3×107cfu/mL的发酵液浸泡小麦种子1 min后,盆栽试验的防效达到56.65%,略高于50%多菌灵WP(1∶100)拌种对照处理的效果。从形态和生理生化特征上可将Kc菌株鉴定为芽孢杆菌属,16S rDNA序列与菌株Bacillus amyloliquefaciens DSM7同源性达99.5%,被鉴定为解淀粉芽孢杆菌。
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| 关 键 词： | 小麦全蚀病 拮抗细菌 16S rDNA 芽孢杆菌 生物防治 |
Screening and Identification of Biocontrol Bacillus Strains against Take-all of Wheat |
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| WU Xian-bing,SUN Rui-kui,WU Yi-xin,WANG Zhi-yuan,WU Xing-xing,MAO Zi-chao,HE Yue-qiu.Screening and Identification of Biocontrol Bacillus Strains against Take-all of Wheat[J].Acta Agriculturae Jiangxi,2012(11):53-55,58. |
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| Authors: | WU Xian-bing SUN Rui-kui WU Yi-xin WANG Zhi-yuan WU Xing-xing MAO Zi-chao HE Yue-qiu |
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| Institution: | 1,2(1.Faculty of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;2.Key Laboratory of Agricultural Biodiversity and Pest Control,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China) |
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| Abstract: | With a disc confrontation assay,234 bacterial isolates antagonistic to Gaeumannomyces graminis var.tritici,the pathogen of wheat take-all disease,have been isolated from the soils in Yunnan province.Among them a strain Kc controlled 56.65% of the disease in the pot experiment when its suspension of 3.3×107 cfu/mL soaked wheat seeds,showing better than 5 g/L Carbendazim.This strain was identified as Bacillus amyloliquefaciens on the basis of its morphological,physiological and biochemical characters,and its 16S rDNA sequence had 99.5% homology with that of Bacillus amyloliquefaciens DSM7. |
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| Keywords: | Take-all of wheat Antagonistic bacterium 16S rDNA Bacillus Biocontrol |
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