| 松辽黑猪H-FABP基因位点多态性研究 |
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| 引用本文： | 高妍,肖书奇,李毅,赵中利,袁宝,张嘉保,赵志辉.松辽黑猪H-FABP基因位点多态性研究[J].中国畜牧兽医,2007,34(3):63-66. |
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| 作者姓名： | 高妍 肖书奇 李毅 赵中利 袁宝 张嘉保 赵志辉 |
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| 作者单位： | 1.吉林大学实验动物中心，长春 130062； 2.吉林大学畜牧兽医学院，长春 130062 |
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| 基金项目： | 国家振兴东北老工业基地科技攻关项目 |
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| 摘 要： | 为检测松辽黑猪H-FABP基因位点多态性，应用PCR-RFLP技术(HinfI、HaeIII和MspI 3种限制性内切酶)检测了62头松辽黑猪5’-上游区和第二内含子的遗传变异情况。结果发现松辽黑猪在MspI多态位点上表现为单一的AA型；而在HinfI多态位点上表现为多态性，等位基因H的基因频率为0.7177，在HaeIII位点上也表现出多态性，等位基因d的基因频率为0.6210。研究结果表明松辽黑猪H-FABP基因5’-上游区和第二内含子存在多态性。
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| 关 键 词： | 松辽黑猪 H-FABP基因 多态性 PCR-RFLP |
| 文章编号： | 1671-7236(2007)03-0063-04 |
| 修稿时间： | 2006年9月8日 |
Study on the Polymorphism of Songliao Black Pig Heart Fatty Acid-Binding Protein Gene |
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| GAO Yan,XIAO Shu-qi,LI Yi,ZHAO Zhong-li,YUAN Bao,ZHANG Jia-bao,ZHAO Zhi-hui.Study on the Polymorphism of Songliao Black Pig Heart Fatty Acid-Binding Protein Gene[J].China Animal Husbandry & Veterinary Medicine,2007,34(3):63-66. |
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| Authors: | GAO Yan XIAO Shu-qi LI Yi ZHAO Zhong-li YUAN Bao ZHANG Jia-bao ZHAO Zhi-hui |
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| Institution: | 1.Laboratory Animal Center, Jilin University, Changchun 130062,China; 2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062,China |
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| Abstract: | In order to detect the polymorphism of heart fatty acid-binding protein (H FABP)in the SongLiao black pig , genetic variations of 5’-upstream region and the second intron in 62 SongLiao black pigs H-FABP gene were checked by PCR RFLP with HinfI, HaeIII and MspI.The results indicate that SongLiao black pig only shows sigle AA genotype in MspI-RFLP, for HinfI-RFLP, SongLiao black pig had polymorphism and frequencies of allele H is 0.7177, and for HaeIII-RFLP, SongLiao black pig had polymorphism and frequencies of allele d is 0.6210.So the experiment indicates that SongLiao black pig has polymorphism in the 5’-upstream region and the second intron of H-FABP gene. |
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| Keywords: | PCR-RFLP |
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