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| 猴免疫缺陷病毒p27蛋白单克隆抗体结合抗原表位的鉴定 | |
| 引用本文： | 李亮,林跃智,那雷,汤艳东,吕晓玲,周建华,曲娟娟.猴免疫缺陷病毒p27蛋白单克隆抗体结合抗原表位的鉴定[J].中国预防兽医学报,2011,33(9). |
| 作者姓名： | 李亮 林跃智 那雷 汤艳东 吕晓玲 周建华 曲娟娟 |
| 作者单位： | 1. 东北农业大学生命科学学院,黑龙江哈尔滨,150030 2. 中国农业科学院哈尔滨兽医研究所 3. 东北农业大学生命科学学院,黑龙江哈尔滨150030;中国农业科学院哈尔滨兽医研究所 |
| 基金项目： | 国家自然科学面上项目(31070809) |
| 摘 要： | 为鉴定抗猴免疫缺陷病毒(SIV)衣壳蛋白p27单克隆抗体(MAb) p27-A5、p27-C7和p27-D2所识别的抗原表位,本研究通过间接ELISA法相加试验对这3株MAbs结合抗原表位进行初步分析,并利用部分重叠的短肽对MAbs的结合抗原表位进行鉴定.结果表明p27-C7和p27-A5的结合抗原表位位于p27蛋白的aa61～aa76(氨基酸序列为61SEGCTPYDINQMLNCV76)；p27-D2的结合抗原表位则位于aa191～aa206(氨基酸序列为191EQTDAAVKNWMTQTLL206).该结果为进一步深入了解p27的抗原特性和建立该蛋白的检测方法奠定了基础. |
| 关 键 词： | 猴免疫缺陷病毒 p27蛋白 单克隆抗体 表位鉴定 |
Identification of monoclonal antibodies against the epitopes on simian immunodeficiency virus p27 capsid protein |
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| LI Liang,LIN Yue-zhi,NA Lei,TANG Yan-dong,LV Xiao-ling,ZHOU Jian-hua,QU Juan-juan.Identification of monoclonal antibodies against the epitopes on simian immunodeficiency virus p27 capsid protein[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(9). | |
| Authors: | LI Liang LIN Yue-zhi NA Lei TANG Yan-dong LV Xiao-ling ZHOU Jian-hua QU Juan-juan |
| Institution: | LI Liang1,LIN Yue-zhi2,NA Lei1,2,TANG Yan-dong2,LV Xiao-ling2,ZHOU Jian-hua2,QU Juan-juan1(1.College of Life Science,Northeast Agricultural University,Harbin 150030,China,2.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
| Abstract: | In this study,three monoclonal antibodies(MAb) against the p27 capsid protein of simian immunodeficiency virus(SIV) were developed,designated p27-A5,p27-C7 and p27-D2.The epitopes on p27 to these MAbs were identified with synthesized short peptides using indirect ELISA.The results showed that MAb of p27-A5 and p27-C7 recognized the same epitop of p27,which was located within sites of aa 61 to aa 76(61SEGCTPYDINQMLNCV76).In addition,the MAb p27-D2 reacted with another epitop of p27 that was located within si... |
| Keywords: | simian immunodeficiency virus p27 protien monoclonal antibody antigenic epitope |
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