| 猴痘病毒荧光定量PCR检测方法的建立 |
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| 引用本文： | 朱娜,陈国强,张敬友,范红结.猴痘病毒荧光定量PCR检测方法的建立[J].南京农业大学学报,2009,32(4). |
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| 作者姓名： | 朱娜 陈国强 张敬友 范红结 |
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| 作者单位： | 1. 南京农业大学动物医学院,江苏 南京,210095
2. 江苏出入境检验检疫局,江苏 南京,210001 |
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| 基金项目： | 国家质检总局科学基金项目 |
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| 摘 要： | 根据GenBank发表的猴痘病毒(AF380138)F3L基因全序列,设计并合成了引物和TaqMan、MGB探针,建立了F3L基因的荧光定量PCR检测方法.应用该方法可从人工合成的猴痘病毒F3L基因片段(48048～48 509 bp)扩增典型的"S"型曲线,25 μL反应体系检测灵敏度可达68拷贝,但不能从鸡痘、山羊痘等病毒中扩增出相应的扩增曲线.结论:本方法可快速、敏感、特异地检测猴痘病毒.
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| 关 键 词： | 猴痘病毒 荧光定量PCR 检测 |
Establishment of real-time PCR assays for detection of monkeypox virus |
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| ZHU Na,CHEN Guo-qiang,ZHANG Jing-you,FAN Hong-jie.Establishment of real-time PCR assays for detection of monkeypox virus[J].Journal of Nanjing Agricultural University,2009,32(4). |
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| Authors: | ZHU Na CHEN Guo-qiang ZHANG Jing-you FAN Hong-jie |
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| Abstract: | In this paper the primers and TaqMan, MGB probes were designed by using Primer Express 3. 0 software according to the entire F3L gene sequence of monkeypox virus in CenBank to develop a real-time PCR assay for rapid identification of monkeypox virus. A typical "S" curve can be got when the artifical F3L gene sequence was used as a target, and the detection limits were 6. 8 copies of target DNA per PCR vial. However, when the chickenpox, goalpox or any other virus were used as targets, a typical " S" curve can not be got. So this assay may represent a battery of tests in detection of monkeypox virus. |
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| Keywords: | monkeypox virus real-time PCR assay detection |
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