| 禽传染性支气管炎病毒鸡胚连续传代后3'端7 kb编码区核酸变异特性 |
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| 引用本文： | 闫宝龙,张晓楠,龚利洋,韩宗玺,邵昱昊,孔宪刚,刘胜旺.禽传染性支气管炎病毒鸡胚连续传代后3'端7 kb编码区核酸变异特性[J].中国预防兽医学报,2010,32(4). |
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| 作者姓名： | 闫宝龙 张晓楠 龚利洋 韩宗玺 邵昱昊 孔宪刚 刘胜旺 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/禽传染病研究室,黑龙江,哈尔滨,150001 |
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| 基金项目： | 国家科技支撑计划重大项目 |
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| 摘 要： | CK/CH/LHLJ/04V在鸡胚上连续传代后,其致病力、免疫原性和组织嗜性均发生了改变。为了研究其生物学特性变化的分子特征,通过RT-PCR的方法扩增出4个不同代次(P3、P40、P70、P110)的毒株的3'端7kb区域,克隆于pMD18-T中进行测序。序列分析表明CK/CH/LHLJ/04V病毒群中含有基因突变的各种亚群。3'端7kb区域的氨基酸替换主要发生在S蛋白。P3和P40中,S1亚基中的氨基酸替换,同样发生在病毒传代过程中的其它亚群中。但是P70和P110中的另一些氨基酸替换却仅发生在某些亚群中。P70和P110的大部分亚群中,3'非编码区出现109bp的缺失,这可能与病毒的复制、转录和致病力有关。病毒3'端7kb区域的变化很可能导致了病毒毒力的减弱、免疫原性的降低和组织嗜性的变化。
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| 关 键 词： | 禽传染性支气管炎病毒 改变 分子特性 3'端7kb区域 |
The characteristics of altered 3'-7 kb regions of an IBV strain after serial passage in embryos |
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| YAN Bao-long,ZHANG Xiao-nan,GONG Li-yang,HAN Zong-xi,SHAO Yu-hao,KONG Xian-gang,LIU Sheng-wang.The characteristics of altered 3''-7 kb regions of an IBV strain after serial passage in embryos[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(4). |
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| Authors: | YAN Bao-long ZHANG Xiao-nan GONG Li-yang HAN Zong-xi SHAO Yu-hao KONG Xian-gang LIU Sheng-wang |
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| Institution: | YAN Bao-long,ZHANG Xiao-nan,GONG Li-yang,HAN Zong-xi,SHAO Yu-hao,KONG Xian-gang,LIU Sheng-wang(Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | The characteristics of 3'-7 kb regions of chicken infectious bronchitis virus(IBV) CK/CH/LHLJ/04V strain were altered after serial passages in chicken embryos. In this study,the 3'-7 kb regions of four passaged IBV(P3,P40,P70 and P110) were amplified by RT-PCR and sequenced. The results showed that the CK/CH/LHLJ/04V virus population contained subpopulations with a mixture of genetic mutants. Deduced amino-acid substitutions were observed in the 3'-7 kb region,primarily in the spike(S) protein. Substitution... |
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| Keywords: | infectious bronchitis virus molecular mechanism 3' -7 kb region |
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