| 鸡MHC-B-F基因外显子3PCR-RFLP-Tas Ⅰ的多态分布 |
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| 引用本文： | 罗庆斌,任广彩,沈栩.鸡MHC-B-F基因外显子3PCR-RFLP-Tas Ⅰ的多态分布[J].中国家禽,2005,27(20):16-18. |
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| 作者姓名： | 罗庆斌 任广彩 沈栩 |
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| 作者单位： | 华南农业大学动物科学学院,广州,五山,510642 |
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| 基金项目： | 本研究由广东省科技计划项目重大专项(2004A2010600)资助 |
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| 摘 要： | 研究利用PCR—RFLP方法检测了MHC-B—F基因外显子3 TasⅠ位点不同基因型在9个鸡品种计235个个体中的分布情况。结果表明MHC—B—F基因外显子3 TasⅠ位点中存在3种基因型，命名为AA、AB、BB。它们在不同品种中的分布各异，AA基因型在杏花鸡、灵山香鸡、丝羽乌骨鸡中的频率显著甚至极显著地高于其它品种（P〈0．05 or P〈0．01），AB基因型在来航鸡与隐性白洛克中的频率相当低（P〈0．05 or P〈0.01），BB基因型在隐性白洛克中的分布频率则极显著地高于其它8个品种（P〈0．01）。
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| 关 键 词： | 鸡 MHC抗原 细胞表面转膜蛋白 B—F基因 多态分布 组织相容性复合体 PCR—RFLP方法 |
| 修稿时间： | 2005年8月29日 |
Polymorphisms of PCR-RFLP-Tas Ⅰ for the Chicken MHC-B-F Gene Exon 3 |
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| Luo Qingbin,Ren Guangcai,Shen Xu.Polymorphisms of PCR-RFLP-Tas Ⅰ for the Chicken MHC-B-F Gene Exon 3[J].China Poultry,2005,27(20):16-18. |
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| Authors: | Luo Qingbin Ren Guangcai Shen Xu |
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| Abstract: | In this study,genotypes of MHC-B-F gene of 235 individuals of 9 different chicken breeds were tested by PCR-RFLP.The results showed that three genotypes,named AA?AB and BB,were found to exist in the Tas I site of the chicken MHC-B-F gene exon 3.The frequency of AA genotype in Xinghua?Lingshan and Silkie was significantly higher(P<0.05) or highly significantly higher (P<0.01) than that of the other breeds.The frequency of AB genotype in two foreign breeds( Leghorn and Recessive White Rock) was very low (P<0.05 or P<0.01).However,the frequency of BB genotype in Recessive White Rock was highly significantly higher (P<0.01) than that of the other 8 breeds. |
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| Keywords: | chicken major histocompatibility complex B-F gene PCR-RFLP |
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