| 新城疫病毒La Sota株F蛋白结构与部分功能区的预测 |
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| 引用本文： | 张晓东,刘怀然,刘培欣,闫丽辉,刘胜旺,孔宪刚.新城疫病毒La Sota株F蛋白结构与部分功能区的预测[J].中国预防兽医学报,2007,29(12):938-942. |
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| 作者姓名： | 张晓东 刘怀然 刘培欣 闫丽辉 刘胜旺 孔宪刚 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/禽传染病研究室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 以新城疫病毒(Newcastlediseace virus,NDV)La sota F蛋白的氨基酸序列为基础,采用Chou-Fasman法、Garnier-Robson法、Karplus-Schulz法与Kyte-Doolittle法、Emini法和Jameson-Wolf法分别预测蛋白质的二级结构和B细胞抗原表位。预测结果表明,NDV La Sota F蛋白结构较为复杂,含有较多的α-螺旋、β-折叠区,转角和无规则卷曲等有柔韧性区域。此外,应用Tmap法预测了NDV La sota F蛋白的跨膜区域,通过同源建模的方法预测了该蛋白的三维空间结构,并比较了强弱毒株裂解位点处空间结构的不同。为进一步通过试验方法确定其蛋白的B细胞表位和从结构出发了解其生物学功能,提供了理论依据。
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| 关 键 词： | F蛋白 结构 B细胞抗原表位 功能 |
| 文章编号： | 1008-0589（2007）12-0938-05 |
| 收稿时间： | 2007-04-15 |
| 修稿时间： | 2007年4月15日 |
Prediction of structure and functional regions for NDV La Sota F protein |
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| ZHANG Xiao-dong,LIU Huai-ran,LIU Pei-xin,YAN Li-hui,LIU Sheng-wang,KONG Xian-gang.Prediction of structure and functional regions for NDV La Sota F protein[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(12):938-942. |
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| Authors: | ZHANG Xiao-dong LIU Huai-ran LIU Pei-xin YAN Li-hui LIU Sheng-wang KONG Xian-gang |
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| Abstract: | The secondary structure and antigenic sites of the fusion protein capsid protein of of newcastle disease vaccine strain(La Sota)was predicted based on published sequences by the methods of of Chou-Fasman,Gamier-Robson and Karplus- Schulz,and Kyte-Doolittle,Emini and Jameson-Wolf.The transmembrane regions of the protein was predicted by the method of Tmap.The three-dimensional structure was obtained by submitting amino acids sequence to the swiss-model server and the differ- ences of the cleavage motifs between velogenic NDV and lentogenic NDV was analyzed.The results showed that the N gene contained several flexible regions such as coils and turns and three membrane spanning regions.Certain epitopes appeared with a high probability of occurrence.This study provided a solid foundation for identification of B cell epitopes for the fusion protein and understanding its biological functions on the basis of protein structure. |
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| Keywords: | NDV La Sota |
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