| 鸡传染性支气管炎病毒核蛋白基因的克隆及在杆状病毒系统中的表达 |
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| 引用本文： | 刘胜旺,孔宪刚,王玮,高磊,童光志,谷守林.鸡传染性支气管炎病毒核蛋白基因的克隆及在杆状病毒系统中的表达[J].中国预防兽医学报,2001,23(6):401-403. |
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| 作者姓名： | 刘胜旺 孔宪刚 王玮 高磊 童光志 谷守林 |
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| 作者单位： | 中国农业科学院 |
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| 摘 要： |
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| 关 键 词： | 鸡传染性支气管炎病毒 核蛋白 基因序列 重组杆状病毒 表达 |
| 文章编号： | 1008-0589(2001)06-0401-03 |
| 修稿时间： | 2001年2月21日 |
Expression of avian infectious bronchitis virus Nucleocapsid by Recombinant
Baculovirus System |
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| LIUSheng_wang,KONGXian_Gang,WANGWei,GAOLei,TONGGuang_zhi,GUShou_lin.Expression of avian infectious bronchitis virus Nucleocapsid by Recombinant
Baculovirus System[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(6):401-403. |
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| Authors: | LIUSheng_wang KONGXian_Gang WANGWei GAOLei TONGGuang_zhi GUShou_lin |
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| Abstract: | With the specific RT_PCR, the nucleocapsid (N) gene of a Infectious Bronchitis Virus(IBV) strain isolated from China was cloned and sequenced. Then a recombinant baculovirus containing the N gene was constructed in order to express the N protein. The expression of N protein in Sf9 cell was determined by Western blot with polyclonal antibodies against IBV. The infected cells were collected at different time post_infection and analyzed by SDS_PAGE. The result showed that the expressed product had a molecular weight of approximately 56kDa and the expression level of the recombinant protein was up to 19.4% of the total cell protein. |
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| Keywords: | Avian infectious bronchitis virus Nucleocapsid Sequene Recombinant Baculovirus System Expression |
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