| 单克隆抗体ELISA检测犊牛粪便中冠状病毒抗原 |
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| 引用本文： | 陈焕春,金梅林.单克隆抗体ELISA检测犊牛粪便中冠状病毒抗原[J].中国兽医杂志,1995,21(1):8-11. |
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| 作者姓名： | 陈焕春 金梅林 |
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| 作者单位： | 华中农业大学畜牧兽医系，武汉市畜牧兽医研究所 |
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| 摘 要： | 用两株单克隆抗体混合后包被ＥＬＬＩＳＡ微孔板，加牛冠状病毒抗原，加经白陶土处理过的兔抗牛冠状病毒免血清，再加上辣根氧化物酶标记的羊抗兔ＩｇＧ抗体，加底物质显色间接ＥＬＩＳＡ检测牛冠状病毒抗原获得成功。用此方法检测细胞培养的牛冠状病毒上清液，其敏感度高出血凝试验（ＨＡ）８倍，从武汉市附近３个奶牛场采集犊牛腹泻粪便１０５份，用单抗ＥＬＩＳＡ检测牛冠状病毒抗原，共检出阳性３６份，并与血凝及血凝抑制试验，
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| 关 键 词： | 冠状病毒 单克隆抗体 酶联免疫吸附 抗原 牛病 |
Detection of Bovine Coronavirus Antigen in Calves Feces by ELISA with Monoclonal Antibodies |
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| Chen Huan Chun,Jin Mei Lin,He Guo Qing,Su Xiong,Chen Yu Chuan.Detection of Bovine Coronavirus Antigen in Calves Feces by ELISA with Monoclonal Antibodies[J].Chinese Journal of Veterinary Medicine,1995,21(1):8-11. |
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| Authors: | Chen Huan Chun Jin Mei Lin He Guo Qing Su Xiong Chen Yu Chuan |
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| Abstract: | ELISA microplates were first coated with the mixture of monoclonal anti-bodies of two cell strains against bovine coronavirus,then the antigen of bovine coronaviruswas added to the wells. Next step Kaolin-treated rabbit immune serum against bovine coronavirus was added as detection antibody,the peroxidase labelled goat anti-rabbit lgG wasadded. Finally the substrate was added.By this indirect ELISA we succeeded in detectingthe antigen of bovine coronavirus;This method was used to detect bovine coronavirus in thesuspension of cell culture,the result showed it was eight times more sensitive than HA. 105fecal samples of diarrheal calves were collected from 3 cow farms near Wuhan City,36 sam-ples were positive. In comparision with HA/HT and electron microscope monoclonal anti-bodies ELISA was simple; quick; highly sensitive and strongly specific. This method over-comes the difficulties of non-specific reaction by only using polyclonal antibodies and thelow OD value by only using a monoclonal antibody. |
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| Keywords: | monoclonal antibodv bovine coronavirus ELISA detection of anti-gen |
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