| 猪传染性胃肠炎病毒重组N蛋白抗原间接ELISA抗体检测方法的建立 |
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| 引用本文： | 孙东波,冯力,时洪艳,刘胜旺,陈洪岩,佟有恩,李伟杰,王明,马思奇,陈建飞.猪传染性胃肠炎病毒重组N蛋白抗原间接ELISA抗体检测方法的建立[J].中国预防兽医学报,2006,28(5):572-576,580. |
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| 作者姓名： | 孙东波 冯力 时洪艳 刘胜旺 陈洪岩 佟有恩 李伟杰 王明 马思奇 陈建飞 |
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| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001
2. 哈尔滨维科生物技术开发公司,黑龙江,哈尔滨,150001 |
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| 摘 要： | 本研究以纯化的原核表达猪传染性胃肠炎病毒N蛋白为诊断抗原，建立了猪传染性胃肠炎病毒抗体检测的间接ELISA诊断方法，将其命名为mTGE-ELISA。该抗原不与其他常见10种猪病的阳性血清发生交叉反应。批内和批间重复性试验的变异系数均小于15％；对仔猪免疫后不同时间的血清检测结果表明mTGE-ELISA与纯化病毒ELISA符合率达95．0％；mTGE-ELISA相对于VN试验的敏感性为96．3％、特异性为92．2％：现地试验中，mTGE-ELISA与Svanova TGEV／PRCV antibody diagnosis Kit的符合率达87．0％，通过中和试验复核结果表明，mTGE-ELISA的假阳性低于Svanova TGEV／PRCV antibody diagnosis Kit。本试验建立的mTGE-ELISA诊断方法具有良好的敏感性和特异性，为免疫猪群抗体监测和TGE流行病学调查提供了一种快速、简便的血清学诊断方法。
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| 关 键 词： | 重组N蛋白 间接ELISA 诊断 |
| 文章编号： | 1008-0589（2006）05-0572-05 |
| 收稿时间： | 2005-07-15 |
| 修稿时间： | 2005-07-15 |
Development of an indirect ELISA for detection antibodies to porcine transmissible gastroenteritis virus using recombinant nucleoprotein antigen |
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| SUN Dong-bo,FENG Li,SHI Hong-yan,LIU Sheng-wang,CHEN Hong-yan,TONG You-en,LI Wei-jie,WANG Ming,MA Si-qi,CHEN Jian-fei.Development of an indirect ELISA for detection antibodies to porcine transmissible gastroenteritis virus using recombinant nucleoprotein antigen[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(5):572-576,580. |
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| Authors: | SUN Dong-bo FENG Li SHI Hong-yan LIU Sheng-wang CHEN Hong-yan TONG You-en LI Wei-jie WANG Ming MA Si-qi CHEN Jian-fei |
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| Institution: | 1. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin 150001, China; 2. Harbin Weike Biotechnology Company,Harbin 150001, China |
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| Abstract: | Using the recombinant and purified N protein as antigen expressed in Escherichia coli BL21,an indirect ELISA was successfully developed to detect antibody to transmissible gastroenteritis virus(TGEV),the indirect ELISA was named rnTGE-ELISA.The recombinant N protein antigen showed no cross-reaction with the positive sera of other ten kinds of swine diseases,coefficient of variability percent(C.V %)of intro-batch duplicativity test and inter-batch duplicativity test was less 15 %;Result of immune piglets were tested with rnTGE-ELISA and purified virus ELISA showed that 95.0 % concordance was obtained;Sensitivity and specificity of rnTGE-ELISA relative to VN was respectively 96.3 % and 92.2 %;in field test rnTGE-ELISA compared with Svanova TGEV/PRCV antibody diagnosis Kit,87.0 % concordance was obtained.Therefore,this rnTGE-ELISA based on recombinant N protein antigen has good sensitivity and specificity,can afforded a simple and rapid means for assessment of vaccination in the field and investigation of TGE epidemiology. |
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| Keywords: | TGEV |
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