| 鸽蛔虫ITS rDNA的克隆与序列分析 |
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| 引用本文： | 李金平,张浩吉,梁详解,蒲文珺,李高强,郭建超,李国清.鸽蛔虫ITS rDNA的克隆与序列分析[J].湖北农业科学,2012(18):4138-4140. |
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| 作者姓名： | 李金平 张浩吉 梁详解 蒲文珺 李高强 郭建超 李国清 |
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| 作者单位： | 华南农业大学兽医学院;佛山科学技术学院 |
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| 基金项目： | 广东省自然科学基金项目(10152800001000007) |
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| 摘 要： | 以采自广东省佛山市的鸽蛔虫(Ascaridia columbae)为研究对象,利用保守引物BD1和BD2扩增鸽蛔虫基因组DNA的ITS rDNA片段,对扩增产物进行克隆和序列测定。结果成功扩增出大小为1045bp的ITS rDNA序列。获得的鸽蛔虫的ITS rDNA序列(AC001、AC002)的5.8S rDNA与GenBank收录的鸡蛔虫5.8S rDNA序列(GenBank登录号为AJ001508)相似性分别为95.5%和94.3%,而与澳大利亚鸽蛔虫5.8 S rDNA序列(GenBank登录号为AJ001509)相似性分别为96.8%和95.5%。将获得的序列与蛔目不同科的代表性蛔虫的5.8SrDNA序列进行比对和系统进化分析,结果表明鸽蛔虫与鸡蛔虫处于同一进化分支上,也表明ITS rDNA是蛔虫分子鉴定的有效遗传标记,这对蛔虫分子分类学及分子流行病学的进一步研究打下了基础。
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| 关 键 词： | 鸽蛔虫(Ascaridia columbae) ITS rDNA 序列分析 系统进化分析 |
Clone and Sequence Analysis of ITS rDNA of Ascaridia columbae |
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| LI Jin-ping,ZHANG Hao-ji,LIANG Xiang-jie,PU Wen-jun,LI Gao-qiang,GUO Jian-chao,LI Guo-qing.Clone and Sequence Analysis of ITS rDNA of Ascaridia columbae[J].Hubei Agricultural Sciences,2012(18):4138-4140. |
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| Authors: | LI Jin-ping ZHANG Hao-ji LIANG Xiang-jie PU Wen-jun LI Gao-qiang GUO Jian-chao LI Guo-qing |
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| Institution: | 1(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China; 2.Foshan University of Science and Technology,Foshan 528231,Guangdong,China) |
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| Abstract: | The internal transcribed spacer(ITS) rDNA of roundworm(Ascaridia columbae) samples collected from Foshan,Guangdong were amplified by PCR using a pair of conserved primers BD1 and BD2.Then the sequence was cloned,sequenced and analyzed.The results showed that the ITS rDNA sequence of A.columbae was 1 045 bp.The similarity of 5.8 S rDNA sequence of A.columbae(AC001,AC002) to that of A.galli(GenBank Accession number,AJ001508) was 95.5% and 94.3% respectively;and to that of Australian A.columbae(GenBank Accession number,AJ001509) was 96.8% and 95.5% respectively.Alignments and phylogenetic analysis of 5.8S rDNA sequences of A.columbae and representative species in different families of Ascaridida showed that A.galli and A.columbae were in the same evolutionary branch.It was proved that ITS rDNA was effective genetic marker of Ascaridida,which layed the foundation for molecular taxonomic and epidemiological research of Ascaridida. |
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| Keywords: | Ascaridia columbae ITS rDNA sequence analysis phylogenetic analysis |
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