| Envision免疫组化技术在诊断猪繁殖与呼吸综合征中的应用 |
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| 引用本文： | 杨鸿,钱君娣,邓桦,何启盖,马春全,卢玉葵.Envision免疫组化技术在诊断猪繁殖与呼吸综合征中的应用[J].中国兽医学报,2010,30(2). |
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| 作者姓名： | 杨鸿 钱君娣 邓桦 何启盖 马春全 卢玉葵 |
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| 作者单位： | 1. 佛山科学技术学院动物医学系,广东,佛山,528231
2. 华中农业大学,动物医学院,湖北,武汉,430070 |
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| 基金项目： | 国际“十一五”科技支撑计划资助项目(2006BAD06-A12); 广东省佛山市科技发展专项资金资助项目(2005020022) |
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| 摘 要： | 为探讨免疫组织化学Envision法在诊断猪繁殖与呼吸综合征(PRRS)中应用的可行性,采用免疫组织化学Envision二步法对30例疑似PRRS病猪肺组织的病毒抗原进行定位、半定量检测。结果表明,Envision法可原位检测病猪肺组织PRRSV抗原的分布;其抗原的阳性表达主要出现在肺巨噬细胞胞浆内,其次为肺泡上皮细胞和细支气管黏膜上皮细胞。依据阳性细胞百分率,进行染色结果判断,检出阳性病猪23例,阳性检出率为76.7%。试验证明,Envision法具有高敏感、低背景、快速简便的特点,可在PRRS诊断中推广应用。
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| 关 键 词： | Envision法 病理诊断 猪繁殖与呼吸综合征 |
Application of Envision immunohistochemical method for detecting of porcine reproductive and respiratory syndrome |
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| YANG Hong,QIAN Jun-di,DENG Hua,HE Qi-gai,MA Chun-quan,LU Yu-kui.Application of Envision immunohistochemical method for detecting of porcine reproductive and respiratory syndrome[J].Chinese Journal of Veterinary Science,2010,30(2). |
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| Authors: | YANG Hong QIAN Jun-di DENG Hua HE Qi-gai MA Chun-quan LU Yu-kui |
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| Institution: | YANG Hong1,QIAN Jun-di1,DENG Hua1,HE Qi-gai2,MA Chun-quan1,LU Yu-kui1 (1.Department of Veterinary Medicine,Foshan University,Foshan,Guangdong 528231,China,2.College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | To observe the value of Envision method of immunohistochemistry techniques for detecting of porcine reproductive and respiratory syndrome (PRRS),the antigens of PRRSV were detected in-situ and semiquantitative by Envision method in lung tissues of 30 suspected PRRS swine.The results showed the PRRSV antigens could be located by Envision method.Positive express of antigens was mostly in the cytoplasm of porcine alveolar macrophages,and was posteriorly in alveolar and bronchiole epithelia.According to the pos... |
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| Keywords: | Envision pathological diagnosis porcine reproductive and respiratory syndrome |
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