| 西南矮马肌肉生长抑制素基因启动子区PCR-RFLP 多态性检测 |
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| 引用本文： | 徐龙鑫,杨胜林,杨海兵,李爱萍,王忠霞.西南矮马肌肉生长抑制素基因启动子区PCR-RFLP 多态性检测[J].中国畜牧兽医,2012,39(9):152-155. |
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| 作者姓名： | 徐龙鑫 杨胜林 杨海兵 李爱萍 王忠霞 |
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| 作者单位： | 1. 贵州大学动物科学院,贵州贵阳,550025
2. 贵州大学动物科学院,贵州贵阳550025;高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州贵阳 550025 |
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| 摘 要： | 利用PCR-RFLP技术对贵州矮马、德保矮马和宁强矮马3个品种的45个个体的肌肉生长抑制素(myostatin, MSTN)基因的启动子区进行多态性分析。结果表明,大小为204 bp的扩增片段经限制性内切酶SspⅠ酶切后,不同基因型经过测序发现156T→C突变,产生AA和AB两种基因型,没有发现BB基因型。贵州矮马和宁强矮马中AB基因型频率大于AA基因型,德保矮马中AA基因型占优势,在3种矮马品种A等位基因频率均大于B等位基因。卡方检测结果发现,贵州和德保矮马在该酶切位点处于平衡状态(P＞0.05),而宁强矮马在该位点分布差异显著(P＜0.05)。
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| 关 键 词： | 西南矮马 MSTN基因 PCR-RFLP 多态性 |
| 收稿时间： | 2012-03-05 |
PCR-RFLP Polymorphism of Promoter Region of MSTN Gene in Southwest Pony |
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| XU Long-xin
,
YANG Sheng-lin
,
YANG Hai-bing
,
LI Ai-ping
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WANG Zhong-xia.PCR-RFLP Polymorphism of Promoter Region of MSTN Gene in Southwest Pony[J].China Animal Husbandry & Veterinary Medicine,2012,39(9):152-155. |
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| Authors: | XU Long-xin YANG Sheng-lin YANG Hai-bing LI Ai-ping WANG Zhong-xia |
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| Institution: | 1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China |
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| Abstract: | In this present study,promoter region of myostation(MSTN) gene of Guizhou pony,Debao pony and Ningqiang pony were analyzed by PCR-RFLP.The results indicated that there was one SspⅠdigestion site in the 204 bp amplified fragment.An T→C mutation at the site 156 bp of this gene by DNA sequencing which produced two sorts of genotypes,AA and AB,however,BB genotype was not founded in this study.The genotype frequency of AB in Guizhou pony and Ningqiang pony were higher than AA,while AA genotype was preponderant in Debao pony.The allele frequency of A were higher than B in all these three pony breeds.Guizhou pony and Debao pony were at Hardy-Weinberg equilibrium(P>0.05),but Ningqiang pony was significantly different(P<0.05). |
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| Keywords: | Southwest pony MSTN gene PCR-RFLP polymorphism |
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