| 一株猪源屎肠球菌的分离、鉴定及益生功能初步研究 |
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| 引用本文： | 王凡,王建设,刘婷,李晓清,王安如.一株猪源屎肠球菌的分离、鉴定及益生功能初步研究[J].中国畜牧兽医,2012,39(7):231-234. |
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| 作者姓名： | 王凡 王建设 刘婷 李晓清 王安如 |
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| 作者单位： | 北京大北农科技集团股份有限公司,饲用微生物工程国家重点实验室,北京 100193 |
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| 基金项目： | 国家973计划项目，北京市海淀区重大科技研发项目，北京市2010年度促进科技成果转化落地区县项目 |
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| 摘 要： | 从健康仔猪粪便中分离到一株乳酸菌ZW002,并从形态、生理生化指标,16S rRNA基因序列分析,鉴定该菌株为屎肠球菌(Enterococcus faecium),将其16S rRNA基因提交GenBank,登录号为JN542513。从耐酸、耐胆盐及抑菌性能等方面对菌株进行益生功能初步研究,结果表明,菌株ZW002表现出良好的耐受性及益菌性能,为猪用微生态制剂的研究开发奠定了基础。
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| 关 键 词： | 益生菌 屎肠球菌 鉴定 益生性能 |
| 收稿时间： | 2011-10-08 |
Isolation,Identification of Enterococcus faecium Strain and Preliminary Study on its Beneficial Effects |
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| WANG Fan
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WANG Jian-she
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LIU Ting
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LI Xiao-qing
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WANG An-ru.Isolation,Identification of Enterococcus faecium Strain and Preliminary Study on its Beneficial Effects[J].China Animal Husbandry & Veterinary Medicine,2012,39(7):231-234. |
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| Authors: | WANG Fan WANG Jian-she LIU Ting LI Xiao-qing WANG An-ru |
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| Institution: | State Key Laboratory of Direct-Fed Microbial Engineering,Beijing Dabeinong Technology Group Co. Ltd.,Beijing 100193,China |
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| Abstract: | In this article,strain ZW002 belonged to Lactobacillus was isolated from healthy pig feces. According to the results of morphological,physiological,biochemical tests,and sequence analysis of 16S rRNA gene,strain ZW002 was identified as Enterococcus faecium,the accession number of 16S rRNA gene is JN542513. Moreover,the preliminary study of its beneficial effects,like acid resistance,bile salt tolerance and antibacterial activity was carried out. The results showed that strain ZW002 exhibited good stress tolerance and antibacterial activity,which lead strain ZW002 to be a promising candidate for probiotic research. |
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| Keywords: | probiotics Enterococcus faecium identification beneficial effects |
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