| 猪FoxO1基因cDNA部分序列的克隆及其组织表达 |
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| 引用本文： | 杨燕军,白亮,庞卫军,杨公社.猪FoxO1基因cDNA部分序列的克隆及其组织表达[J].畜牧兽医学报,2007,38(11):1143-1148. |
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| 作者姓名： | 杨燕军 白亮 庞卫军 杨公社 |
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| 作者单位： | 西北农林科技大学,动物脂肪沉积与肌肉发育实验室,杨凌,712100 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目(2004CB117506) |
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| 摘 要： | 根据人、黑猩猩及大鼠等物种FoxO1基因同源序列设计引物,利用RT-PCR方法从猪肝脏中克隆FoxO1基因cDNA的部分序列,组织特异性表达分析表明,FoxO1基因在1日龄和9月龄猪的肝、肺、肾、脾、心、胃、皮下脂肪、内脏脂肪、背最长肌和股四头肌等组织中均表达,只是表达丰度随发育阶段和组织的不同有所差异。1日龄猪的内脏脂肪中FoxO1的表达丰度最高,心脏和骨骼肌中相对较低;而9月龄猪的脾脏中FoxO1相对表达丰度最高,明显高于免疫机能尚未完全建立的初生猪,显示出FoxO1可能在机体的免疫调节中起一定作用,另外,9月龄猪的FoxO1表达丰度不仅在平滑肌和骨骼肌中有显著差异,而且在不同类型的骨骼肌中也存在显著差异,显示出FoxO1的表达可能与骨骼肌类型和运动强度有关。
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| 关 键 词： | 猪 FoxO1基因 克隆 组织表达 |
| 文章编号： | 0366-6964（2007）11-1143-06 |
| 修稿时间： | 2006-11-22 |
Partial cDNA Cloning and Tissue Expression of FoxO1 in Pig |
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| YANG Yan-jun,BAI Liang,PANG Wei-jun,YANG Gong-she.Partial cDNA Cloning and Tissue Expression of FoxO1 in Pig[J].Acta Veterinaria et Zootechnica Sinica,2007,38(11):1143-1148. |
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| Authors: | YANG Yan-jun BAI Liang PANG Wei-jun YANG Gong-she |
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| Institution: | Laboratory of Animal Fat Deposition and Muscle Development, Northwest Agriculture and Forestry University, Yangling 712100, China |
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| Abstract: | The partial cDNA of FoxO1 was cloned from pig's liver by RT-PCR,and primer was designed according to the homology blast among human,chimpanzee and rat.Analysis of tissue expression showed that FoxO1 was widely expressed in the liver,lung,kidney,spleen,heart,stomach,subcutaneous adipose,visceral adipose,m.longissimus dorsi and m.quadriceps femoris.Moreover,FoxO1 expressional profile was different between in 1-day-old and 9-month-old pigs following the different developmental stage and tissues.FoxO1 was highest expressed in visceral adipose of 1-day-old pigs while lower in heart and skeletal muscle.Furthermore,FoxO1 had the highest expression in spleen of 9-month-old pigs and significantly higher than piglet that didn't have complete immunity ability,showing FoxO1 may be involved in body immunomodulation.In addition,the expression profile of FoxO1 not only was significantly different from smooth muscle and skeletal muscle,but also was significantly different from various skeletal muscle of 9-month-old pig.It implied that the expression of FoxO1 is intimately related to skeletal muscle types and exercise intensity. |
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| Keywords: | pig FoxO1 gene clone tissue expression |
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