| 油松猝倒病病原菌鉴定及致病性测定 |
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| 引用本文： | 张茹琴.油松猝倒病病原菌鉴定及致病性测定[J].安徽农业科学,2010,38(20):10768-10769,10782. |
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| 作者姓名： | 张茹琴 |
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| 作者单位： | 青岛农业大学农学与植物保护学院,山东青岛,266109 |
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| 基金项目： | 青岛农业大学高层次人才启动基金项目 |
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| 摘 要： | 目的]油松苗期发生的猝倒病是油松育苗工作的极大障碍,对该病害病原菌的正确鉴定是研究其致病机理、开展防治工作等的基础。方法]对1株引起油松苗猝倒病的病原真菌通过形态学、培养性状、致病性及rDNA ITS序列测定来鉴定该病原菌。结果]在PDA培养基上,该病原菌幼嫩菌丝无色,但在生长过程中逐渐变为褐色,部分菌丝膨大成念珠状;菌丝细胞多核;菌丝平均直径6~10μm,分支发生点附近缢缩并形成一隔膜,分支与再分支呈直角;不产生无性孢子,也不产生菌核;能引起油松苗猝倒病;其rDNAITS序列与瓜亡革菌（Thanatephorus cucumeris）的2个菌株的相似性最大,均为97%。结论]该病原菌被鉴定为立枯丝核菌（Rhizoctonia solaniKhn）。该研究可为后续试验的进行奠定基础。
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| 关 键 词： | 油松 形态学 致病性 rDNA ITS序列 立枯丝核菌 |
Identification of a Pathogen Causing Pinus tabulaeformis Seedling Damping-off |
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| ZHANG Ru-qin.Identification of a Pathogen Causing Pinus tabulaeformis Seedling Damping-off[J].Journal of Anhui Agricultural Sciences,2010,38(20):10768-10769,10782. |
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| Authors: | ZHANG Ru-qin |
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| Institution: | ZHANG Ru-qin(College of Agronomy and Plant Protection,Qingdao Agricultural University,Qingdao,Shandong 266109) |
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| Abstract: | Objective] Pinus tabulaeformis damping-off has brought serious losses to P.tabulaeformis seedling and reforestation,a correct identification of the pathogens of damping-off is a basis for studying the pathogenic mechanism and controlling the disease.Methods]In this study,a pathogenic fungus of P.tabulaeformis seedling damping-off was identified by investigating the pathogenic fungus morphology,cultural characteristics,pathogenicity and rDNA ITS sequence analysis.Result] Young hyphae of the fungus was colourless,it produced white to brown mycelium when grown on PDA medium.Its hyphal cells were multinucleate,the hyphae were 6-10 μm wide and tended to branch at right angles,a septum was near each hyphal branch and a slight constriction at the branch;it did not produce spores and sclerotia;it could cause P.tabulaeformis seedling damping-off;its rDNA ITS sequences had highest similarity with two strains of Thanatephorus cucumeris,both similarity were 97%.Conclusion] The pathogen was identified as Rhizoctonia solani Kühn.This study laid a foundation for the future researches. |
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| Keywords: | Pinus tabulaeformis Morphology Pathogenicity rDNA ITS sequences Rhizoctonia solani Kühn |
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