| 一株海洋石油烃降解菌的分离鉴定及特性研究 |
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| 引用本文： | 周常义,;林情员,;苏国成,;庄宏儒,;方少华,;苏文金.一株海洋石油烃降解菌的分离鉴定及特性研究[J].厦门水产学院学报,2009(1):24-28. |
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| 作者姓名： | 周常义 ;林情员 ;苏国成 ;庄宏儒 ;方少华 ;苏文金 |
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| 作者单位： | [1]集美大学生物工程学院,福建厦门361021; [2]厦门市食品生物工程技术研究中心,福建厦门361021; [3]厦门市水产研究所,福建厦门361005; [4]福建省海洋研究所,福建厦门361012 |
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| 基金项目： | 福建省科技重点项目（2006N0013）;福建省科技厅项目（2007F5077） |
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| 摘 要： | 在福建省厦门市集美嘉庚公园旁的码头，从受污染的海水中筛选出一株能以0#柴油为唯一碳源的石油降解菌JMUXMS-100，通过生理生化鉴定和16SrDNA同源性序列分析，鉴定该菌为不动杆菌属（Acinetobacter sp．）．实验研究了时间、底物浓度、pH值和温度对该菌生长和降解率的影响，结果表明，降解率随时间的延长而增大，随着底物浓度的上升而降低．最佳初始pH值为7．0，最适生长温度为28℃．经3d培养，对质量浓度为100—500mg／L的柴油降解率为38．7％～57．2％．
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| 关 键 词： | 石油烃降解菌 不动杆菌 16S rDNA 生物学特性 降解率 |
Research on the Isolation,Identification and Characteristics of a Marine Hydrocarbon Degrading Bacteria |
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| Institution: | ZHOU Chang-yi,LIN Qing-yuan , SU Guo-cheng, ZHUANG Hong-ru, FANG Shao-hua, SU Wen-jin(1.School of Biotechnology Engineering, Jimei University, Xiamen 361021, China; 2. Food Bio-engineering Research Center of Xiamen, Xiamen 361021, China;3. Xiamen Institute of Fishery, Xiamen 361005, China; 4. Fujian Institute of Oceanology, Xiamen 361012, China) |
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| Abstract: | A hydrocarbon degrading bacteria strain JMUXMS - 100 was isolated from the oily sea water with 0# diesel oil as the sole carbon source. Through biochemical-physiological identification and sequence analysis of the 16S rDNA, the strain was identified as Acinetobacter sp. The influence of growth date, substrate concentration, the quantity of inoculation and pH on degrading ability and growth of JMUXMS - 100 strain were studied. The results showed that oil degrading rate time and decreased with oil concentration. The optimal pH and 7.0 and temperature 28 ~C. After 3 days, the oil degrading of JMUXMS - 100 strain increased with the temperature for the growth of the strain were rate of JMUXMS- 100 strain was between 38.7 % and 57.2 % according to 100 -500 mg/L oil. |
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| Keywords: | hydrocarbon degrading bacteria Acinetobacter sp 16S rDNA biological characteristics degrading rate |
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