| 党参茎基腐病镰孢菌鉴定及其对杀菌剂的敏感性研究 |
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| 引用本文： | 漆永红,李敏权,曹素芳,蒋晶晶,李雪萍,李青青,徐美蓉,李继平.党参茎基腐病镰孢菌鉴定及其对杀菌剂的敏感性研究[J].农学学报,2021,11(2):74-78. |
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| 作者姓名： | 漆永红 李敏权 曹素芳 蒋晶晶 李雪萍 李青青 徐美蓉 李继平 |
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| 作者单位： | 1.甘肃省农业科学院植物保护研究所,兰州 730070;2.甘肃省农业科学院林果花卉研究所,兰州 730070;3.甘肃省农业科学院农业质量标准与检测技术研究所,兰州 730070 |
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| 基金项目： | 甘肃省农业科学院科研条件建设及成果转化项目“黄芩根腐病害关键防控技术研究”(2020GAAS25);国家重点研发计划“特色经济作物化肥农药减施技术集成研究与示范”(2018YFD0201100);甘肃省重点人才项目“党参根腐病综合防控技术集成、示范推广与人才培养”(2019RCXM072);嘉峪关市科技计划“嘉峪关市设施蔬菜根腐病综合治理技术研究与应用”(18-20) |
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| 摘 要： | 为明确党参茎基腐病害的病原、致病性和优势病原种类及其对不同杀菌剂的敏感性,采用组织分离法进行病原菌分离,并根据其培养性状、形态学和分子生物学特征进行鉴定,同时采用平皿菌丝生长抑制法测定不同药剂的敏感性。结果表明,该病害的病原为尖孢镰孢菌(Fusarium oxysporum)、茄病镰孢菌(F. sonali)和木贼镰孢菌(F. equiseti),其中尖孢镰孢菌为优势种。室内致病性测试结果表明,3种镰孢菌均可引起党参茎基腐病,其中尖孢镰孢菌的致病性最强,显著高于其他2种菌。供试药剂对菌丝生长均有抑制作用,苯醚甲环唑对3种镰孢菌具有很强的抑制活性,同时3种镰孢菌对吡唑醚菌酯的敏感性较高。
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| 关 键 词： | 党参 茎基腐病 镰孢菌 病原鉴定 杀菌剂 敏感性测定 |
| 收稿时间： | 2020-05-19 |
Identification of Codonopsis pilosula Crown Rot Disease and Its Sensitivity to Fungicides |
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| Qi Yonghong,Li Minquan,Cao Sufang,Jiang Jingjing,Li Xueping,Li Qingqing,Xu Meirong,Li Jiping.Identification of Codonopsis pilosula Crown Rot Disease and Its Sensitivity to Fungicides[J].Journal of Agriculture,2021,11(2):74-78. |
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| Authors: | Qi Yonghong Li Minquan Cao Sufang Jiang Jingjing Li Xueping Li Qingqing Xu Meirong Li Jiping |
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| Institution: | 1.Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China;2.Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China;3.Institute of Agricultural Quality Standards and Testing Technology, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China |
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| Abstract: | To clarify the pathogen, pathogenicity and dominant pathogen species of Codonopsis pilosula crown rot disease and their sensitivity to different fungicides, the pathogen was isolated by tissue isolation method and identified according to its culture traits, morphology and molecular biological characteristics. Meanwhile, the sensitivity of different fungicides was determined by mycelium growth inhibition method. The results showed that the disease pathogens were identified as Fusarium oxysporum, F. sonali and F. equiseti. The dominant species was F. oxysporum. Pathogenicity testing showed that the three species could cause C. pilosula crown rot disease, F. oxysporum had the strongest pathogenicity, and its disease rate and disease index had a significant difference from those of the other two. Laboratory toxicity test of different fungicides to the mycelia growth of the three species showed that difenoconazole fungicide had the strongest inhibition activity, and the three Fusarium species had the highest sensitivity to pyraclostrobin fungicide. |
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| Keywords: | Codonopsis pilosula Crown Rot Disease Fusarium spp Pathogen Identification Fungicides Sensitivity Testing |
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