| 犬瘟热病毒单克隆抗体的制备及鉴定 |
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| 引用本文： | 孙玮,王铁成,杨松涛,高玉伟,王承宇,张涛,杨玉姣,刘玉秀,阮洋,靳晓霞,夏咸柱.犬瘟热病毒单克隆抗体的制备及鉴定[J].动物医学进展,2011,32(3):73-77. |
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| 作者姓名： | 孙玮 王铁成 杨松涛 高玉伟 王承宇 张涛 杨玉姣 刘玉秀 阮洋 靳晓霞 夏咸柱 |
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| 作者单位： | 孙玮,杨玉姣,刘玉秀,SUN Wei,YANG Yu-jiao,LIU Yu-xiu(吉林大学畜牧兽医学院,吉林长春,130062);王铁成,杨松涛,高玉伟,王承宇,夏咸柱,WANG Tie-cheng,YANG Song-tao,GAO Yu-wei,WANG Cheng-yu,XIA Xian-zhu(解放军军事兽医研究所,吉林长春,130062;吉林省人兽共患病预防与控制重点实验室,吉林长春,130062);张涛,阮洋,靳晓霞,ZHANG Tao,RUAN Yang,JIN Xiao-xia(解放军军事兽医研究所,吉林长春,130062) |
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| 摘 要： | 将纯化的犬瘟热病毒(CDV)分别与弗氏完全佐荆(CFA)和弗氏不完全佐剂(IFA)乳化制备的乳化抗原作为免疫原,以有限稀释法和间接ELISA法进行单克隆抗体的筛选,从而获得了3株稳定分泌单克隆抗体的杂交瘤细胞株2F7、3G2、5E3.亚型鉴定结果显示,2F7为IgG2a型,3G2为IgGl,5E3为IgGM.用2F7制...
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| 关 键 词： | 犬瘟热病毒 杂交瘤 单克隆抗体 |
Preparation and Identification of Monoclonal Antibodies Against Canine Distemper Virus |
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| SUN Wei,WANG Tie-cheng,YANG Song-tao,GAO Yu-wei,WANG Cheng-yu,ZHANG Tao,YANG Yu-jiao,LIU Yu-xiu,RUAN Yang,JIN Xiao-xia,XIA Xian-zhu.Preparation and Identification of Monoclonal Antibodies Against Canine Distemper Virus[J].Progress In Veterinary Medicine,2011,32(3):73-77. |
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| Authors: | SUN Wei WANG Tie-cheng YANG Song-tao GAO Yu-wei WANG Cheng-yu ZHANG Tao YANG Yu-jiao LIU Yu-xiu RUAN Yang JIN Xiao-xia XIA Xian-zhu |
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| Institution: | SUN Wei1,WANG Tie-cheng2,3,YANG Song-tao2,GAO Yu-wei2,WANG Cheng-yu2,ZHANG Tao2,YANG Yu-jiao1,LIU Yu-xiu1,RUAN Yang2,JIN Xiao-xia2,XIA Xian-zhu2,3(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin,130062,China,2.Institute of Military Veterinary,AMMS,3.Key Laboratory of Jilin Province for Zoonosis Prevention and Control,China) |
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| Abstract: | With the depurated CDV and emulsified antigens of CFA and IFA as immunogen,use the limiting dilution assay and indirect ELISA for selecting,three hybridoma cell strains stably producing monoclonal antibodies against CDV which named 2F7,3G2,5E3 were obtained.Immunoglobulin subtype experiments showed that 2F7,3G2 and 5E3 were pertaining to IgG2a,IgG1and IgM,respectively.2F7,which conjugated with FITC,was confirmed to have good specificity by direct immunofluorescence assay based on the detection for the Vero ... |
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| Keywords: | Canine distemper virus hybridoma monoclonal antibody |
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