| J 亚群禽白血病病毒gp85单抗1E3株抗原识别表位的初步分析 |
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| 引用本文： | 莫虹斐,余多,孙淼,陈福勇,曹红.J 亚群禽白血病病毒gp85单抗1E3株抗原识别表位的初步分析[J].中国兽医杂志,2011,47(12). |
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| 作者姓名： | 莫虹斐 余多 孙淼 陈福勇 曹红 |
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| 作者单位： | 1. 中国农业大学动物医学院,北京 海淀 100193 西北农林科技大学动物医学院,陕西 杨凌 712100
2. 中国农业大学动物医学院,北京 海淀,100193 |
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| 基金项目： | 国家自然科学基金(U0831002) |
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| 摘 要： | J 亚型禽白血病是由 J 亚群禽白血病病毒引起的一种以成髓细胞增生、免疫抑制、生长发育受阻为特点的传染性肿瘤疾病,于1988年首次发现,其病毒囊膜表面蛋白gp85决定了病毒亚群特异性.本试验将原核表达质粒pET-28a-J gp85中的gp85基因分段亚克隆至重组表达质粒pGEX-6p-1上并在大肠杆菌中成功地表达,用ALV-J Sl gp85单克隆抗体1E3株对分段表达的gp85蛋白进行初步的免疫印迹分析.结果表明:ALV-J Sl gp85单抗1E3株所识别的抗原表位位于蛋白N端的第69至112个氨基酸之间.这将为ALV-J gp85抗原决定簇所在位点及其免疫学功能的研究奠定基础.
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| 关 键 词： | J 亚群禽白血病病毒 gp85蛋白 单克隆抗体 抗原表位 |
Preliminary analysis of avian leukosis virus subgroup J gp85 antigenic epitope recognized by monoclonal antibody 1E3 |
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| MO Hong-fei
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YU Duo
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SUN Miao
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CHEN Fu-yong
,
CAO Hong.Preliminary analysis of avian leukosis virus subgroup J gp85 antigenic epitope recognized by monoclonal antibody 1E3[J].Chinese Journal of Veterinary Medicine,2011,47(12). |
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| Authors: | MO Hong-fei YU Duo SUN Miao CHEN Fu-yong CAO Hong |
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| Institution: | MO Hong-fei1,2,YU Duo1,SUN Miao1,CHEN Fu-yong1,CAO Hong1(1.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China,2.College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China) |
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| Abstract: | Subgroup J avian leukosis virus(ALV-J) can cause a marrow cell proliferation,immune suppression,retarded growth and tumor.In this study,the gp85 gene of avian leukosis virus subgroup J S1 strain isolated in China was sub-cloned into the expression vectors pGEX-6p-1 for constructions of expression plasmids and successfully expressed in E.coli.J-gp85 was dissected into several overlapping epitopes,which were used to react with McAbs by Western-blot.The results showed that the 69th to 112th amino acids in the ... |
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| Keywords: | ALV-J envelope protein gp85 monoclonal antibodies antigenic epitopes |
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