| 嗜水气单胞菌的分离与16S rDNA鉴定 |
| |
| 引用本文： | 柳桐,王生奎,孙亭亭,马志亮,秦波,张以芳,信爱国.嗜水气单胞菌的分离与16S rDNA鉴定[J].安徽农业科学,2014(13):3907-3910. |
| |
| 作者姓名： | 柳桐 王生奎 孙亭亭 马志亮 秦波 张以芳 信爱国 |
| |
| 作者单位： | 云南农业大学动物科学技术学院;云南省畜牧兽医科学院; |
| |
| 基金项目： | 云南省应用基础研究重点项目(2011FA014);云南省科技创新人才计划项目(2011HB035);国家自然科学基金项目(31260598) |
| |
| 摘 要： | 目的]分离并鉴定鱼嗜水气单胞菌,并构建系统发育分析.方法]从云南某鱼场采集发病鱼的病变组织进行细菌分离与培养,并对分离菌株进行初步鉴定、16S rDNA鉴定和系统发育分析.结果]分离培养到1株细菌,命名为Ah1305.经表型鉴定、生化试验及16S rDNA系统发育分析,鉴定为嗜水气单胞菌.结论]该研究可为快捷、准确检测鱼嗜水气单胞引起的疾病提供参考.
|
| 关 键 词： | 嗜水气单胞菌 分离鉴定 16S rDNA |
Isolation and 16S rDNA Identification of Aeromonas hydrophila |
| |
| LIU Tong;XIN Ai-guo.Isolation and 16S rDNA Identification of Aeromonas hydrophila[J].Journal of Anhui Agricultural Sciences,2014(13):3907-3910. |
| |
| Authors: | LIU Tong;XIN Ai-guo |
| |
| Institution: | LIU Tong;XIN Ai-guo;College of Animal Science and Technology,Yunnan Agricultural University;Yunnan Academy of Animal Husbandry and Veterinary Sciences; |
| |
| Abstract: | Objective] The aim was to isolate and identify Aeromonas hydrophila and establish the phylogeny.Method] Lesion tissue of fish was collected from a certain farm in Yunnan Province,and then the isolated strain was identified by preliminary identification,16S rDNA identification and phylogenetic analysis.Result] The bacteria were isolated and named as Ah1305.Through the morphological identification,biochemical test and 16S rDNA phylogeny analysis,it was identified as Aeromonas hydrophila.Conclusion] The study can provide reference for quick and accurately test the disease caused by Aeromonas hydrophila. |
| |
| Keywords: | Aeromonas hydrophila Isolation and identification 16S rDNA |
| 本文献已被 CNKI 维普 等数据库收录！ |
