| 供体细胞的不同处理及重组胚化学激活对体细胞克隆胚胎发育的影响综述 |
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| 引用本文： | 李晟阳,罗光彬,薛可,纪英卓,孙明亮.供体细胞的不同处理及重组胚化学激活对体细胞克隆胚胎发育的影响综述[J].畜禽业,2005(9):37-39. |
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| 作者姓名： | 李晟阳 罗光彬 薛可 纪英卓 孙明亮 |
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| 作者单位： | 沈阳农业大学畜牧兽医学院,辽宁,沈阳,110161 |
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| 摘 要： | 哺乳动物体细胞克隆经过几年的发展,已取得了很大进展。已有多种细胞被采用并得到了克隆后代。但动物克隆效率仍然很低,供体细胞的选择和重组胚激活是动物克隆中的重要步骤。供体细胞的细胞周期、细胞类型、冷冻处理和化学激活等方面都可能影响动物克隆的效率。
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| 关 键 词： | 核移植 供体细胞 化学激活 |
| 文章编号： | 1008-0414（2005）09-0037-03 |
| 收稿时间： | 2005-07-24 |
| 修稿时间： | 2005年7月24日 |
The influence of different treatment with donor cells and chemical activation on the development of embryoes drived from somatic cells |
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| LI Sheng-yang,LUO Guang-bin,XUE Ke,JI Ying-zhuo,SUN Ming-liang.The influence of different treatment with donor cells and chemical activation on the development of embryoes drived from somatic cells[J].Livestock and Poultry Industry,2005(9):37-39. |
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| Authors: | LI Sheng-yang LUO Guang-bin XUE Ke JI Ying-zhuo SUN Ming-liang |
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| Abstract: | Although much progress has been achieved in the last few years , mammalian cloning is still inefficient . Donor cell selection and reconstructed embryo activation is critical fur the success of cloning animal, and they are also important factors affecting the cloning efficiency. The effect of using donor cells of different cell cycle, cell type, chemical activation, as well as refrigeration ,on cloning animal has been summarized. |
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| Keywords: | nuclear transfer donor cell chemical activation |
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