| 丁鱥雌核发育鱼的异源精子诱导及其与亲本的RAPD比较分析 |
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| 引用本文： | 凌去非.丁鱥雌核发育鱼的异源精子诱导及其与亲本的RAPD比较分析[J].水产学报,2005,29(1):120-123. |
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| 作者姓名： | 凌去非 |
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| 作者单位： | [1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室，上海200090 [2]新疆生产建设兵团农十师额河特种鱼类繁育场，新疆北屯836000 |
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| 基金项目： | 新疆生产建设兵团科委农业科学研究与技术开发项目(NKB02N10NK16XM) |
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| 摘 要： | Gynogenesis is thought to be a useful method to generate fully inbred line and to initiate monosex culture in teleost fish. This article presents results of a study of gynogenesis of tench, Tinca tinca L. from Eltrix River of Xinjiang, China, induced by the common carp ( Cyprinus carpio ) sperm inactivated with UV irradiation. When cold shocking method was used to prevent exmasion of the second polar body in order to produce gynogenetic diploids, the optimum treated parameters were screened as cold shocking for 20min at 4℃, 5min after insemination. On this condition, 10.23% of the treated eggs survived at feeding stage. A comparative study between the gynogenesis and their parents was made by RAPD technique. 82 random primers of 10 nucleotide long sequence were used in RAPD analysis. The results showed that each primer gives 3- 12 fragments for each sample with the fragment length 330 - 2 460 bp, and the genetic similarity between gynogenesis and their mother was 97.4 %, while the genetic similarity between gynogenesis and their father was 27.4%, and there were no bands only shared by gynogenesis and their father, the common carp. It revealed that the chromosome of their father, the common carp, did not penetrate into the construction of gynogenetic tench.
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| 关 键 词： | 丁鱥 雌核发育 随机扩增多态DNA |
| 文章编号： | 1000-0615(2005)01-0120-04 |
| 收稿时间： | 2014/3/13 0:00:00 |
| 修稿时间： | 2003年8月26日 |
The inducing of gynogenetic tench by heterologous sperm and comparison with parents by RAPD |
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| LING Qu-fei.The inducing of gynogenetic tench by heterologous sperm and comparison with parents by RAPD[J].Journal of Fisheries of China,2005,29(1):120-123. |
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| Authors: | LING Qu-fei |
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| Institution: | Key Labor atory of Aquatic Genetic Resources and Aquaculture Ecosy stem Certificated by the Ministry of Agriculture, Shanghai Fisheries University , Shanghai 200090, China; |
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| Abstract: | |
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| Keywords: | Tinca tinca gynogenesis random amplified polymorphic DNA( RAPD) |
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