| 转Hu-IFN-α基因草鱼雌核发育F1的遗传配型分析 |
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| 引用本文： | 肖调义.转Hu-IFN-α基因草鱼雌核发育F1的遗传配型分析[J].水产学报,2006,30(6):837-842. |
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| 作者姓名： | 肖调义 |
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| 作者单位： | 湖南农业大学动物科技学院 |
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| 基金项目： | 国家863计划中试项目(NO：101-01-05) |
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| 摘 要： | The Hu-IFN-α gene, which was transducted into downstream promoter of β-actin gene of common carp (Cyprinus carpio), was recombined by DNA recombination technology. These recombined genes were injected into 1-2 cell fertilized eggs of grass carp (Ctenophatyngodon idellus) by microinjection technology, we gained transgenetic fish by molecular detection methods. In order to analyse the genetic expression of tranHu-IFN-α gene gynogensis F1, which male individualization were gained by raising methyltestosterone, molecular genetic marker technology was used. In our research, 30 random primers were picked out from 48 and were used into RAPD-PCR, the result indicated that 1 169 clear, steady and repeated DNA finger printing bands were achieved. On the basis of gentic distance matrix among tranHu-IFN-α gene gynogenesis F1 group, the genetic relationship of gynogenesis F1 were analysed by UPGMA, the results showed the genetic patterns are close between the 3# male gynogenesis F1 and the the 23# female of gynogenesis F1, 5# and 27#, 2# and 28#, 2# and 30#. The data indicated that these group could be served as parent of tranHu-IFN-α grass carp (Ctenophatyngodon idellus) pure line.
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| 关 键 词： | 转基因草鱼 Hu-IFN-α基因 雌核发育 遗传配型 RAPD |
| 文章编号： | 1000-0615（2006）06-0837-06 |
| 收稿时间： | 5/5/2008 12:00:00 AM |
| 修稿时间： | 5/5/2008 12:00:00 AM |
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