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刀鲚精子超微结构研究
引用本文:王冰,万全,李飞,庄永龙,沈保平.刀鲚精子超微结构研究[J].水利渔业,2010(3).
作者姓名:王冰  万全  李飞  庄永龙  沈保平
作者单位:安徽农业大学动物科技学院;安徽大学生物技术中心;安徽省长江水生动物保护研究中心;
基金项目:安徽省科学技术厅项目(07030503049)
摘    要:在光学显微镜及透射电子显微镜下观察刀鲚精子超微结构,并对精子各部分长度进行测定。研究表明,刀鲚精子由头部、中段和尾部(鞭毛)组成。头部在光学显微镜下近梭形,透射电镜下纵切则近圆形。头部无顶体,由细胞核组成,核内染色质致密,空隙少,几乎无细胞质。头部后端偏一侧处有一植入窝,内有中心粒复合体。精子中段位于核后端,由中心粒复合体和袖套组成。中心粒复合体由近端中心粒和远端中心粒组成,两者基本位于一直线上。袖套肥厚的一侧有较多的线粒体和囊泡。尾部分为主段和末段,无侧鳍。主段具典型的9+2的轴丝结构,末段很短,无典型轴丝结构。通过光学显微镜测定,精子头部为(2.34±0.16)μm,中段(1.49±0.18)μm,头宽(1.29±0.21)μm,尾部长(34.07±4.31)μm,全长(37.77±4.21)μm。

关 键 词:刀鲚  精子  超微结构  

Study on the Ultrastructure of Coilia ectenes Spermatozoon
WANG Bing,WAN Quan,LI Fei,ZHUANG Yong-long,SHEN Bao-ping.Study on the Ultrastructure of Coilia ectenes Spermatozoon[J].Reservoir Fisheries,2010(3).
Authors:WANG Bing  WAN Quan  LI Fei  ZHUANG Yong-long  SHEN Bao-ping
Institution:WANG Bing1,WAN Quan1,LI Fei1,ZHUANG Yong-long2,SHEN Bao-ping3 (1. College of Animal Science , Technology,Anhui Agricultural University,Hefei 230036,2. Biotechnology Center,AnhuiUniversity,Hefei 230039,3. Anhui Provincial Changjiang River Aquatic Animal Protection , Research Center,Wuwei 238300)
Abstract:Ultrastructure of Coilia ectenes spermatozoon was studied with light microscope(LM) and transmission electron microscope(TEM),and the lengths of different parts of spermatozoon were measured under LM. The results showed that the spermatozoon of Coilia ectenes could be mainly divided into three parts: head,midpiece and tail (flagellum). Head of spermatozoon was spindle-like shaped under LM and spherical shaped under TEM when longitudinally sectioned. Head was mainly composed of the nucleus,and without acroso...
Keywords:Coilia ectenes  Spermatozoon  Ultrastructure  
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