| 日本黄姑鱼精子生理特性及超低温冷冻保存研究 |
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| 引用本文： | 闫文罡,章龙珍,庄平,刘鹏,江琪.日本黄姑鱼精子生理特性及超低温冷冻保存研究[J].海洋渔业,2008,30(2). |
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| 作者姓名： | 闫文罡 章龙珍 庄平 刘鹏 江琪 |
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| 作者单位： | 1. 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业重点开放实验室,上海,200090;上海水产大学生命学院,上海,200090
2. 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业重点开放实验室,上海,200090 |
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| 基金项目： | 科技部科技基础条件平台建设计划，农业部重点实验室基金 |
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| 摘 要： | 对日本黄姑鱼精子部分生理特性及其超低温冷冻保存技术进行了研究。结果表明,日本黄姑鱼精液pH值为7.0~7.5,精子密度为(11.23±2.78)×109/m l,精子寿命(229.33±17.16)s。在盐度为30~35,pH为7.5~8.5时,精子的活力最高,分别达到(88.33±2.89)%和(88±4.33)%。精子在室温(25℃)条件下,可存活24 h;在低温(4℃)条件下可以存活36 h。以D液作为稀释液,20%的乙二醇作为抗冻剂,利用快速降温法对精子进行超低温冷冻保存,38℃水浴快速解冻,解冻后的精子获得最高的活力为(47±5.78)%。
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| 关 键 词： | 日本黄姑鱼 精子 活力 稀释液 抗冻剂 |
Study on physiological characteristics and cryopreservation of spermatozoa in Japanese croaker(Argyrosomus japonicus) |
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| YAN Wen-gang,ZHANG Long-zhen,ZHUANG Ping,LIU Peng,JIANG Qi.Study on physiological characteristics and cryopreservation of spermatozoa in Japanese croaker(Argyrosomus japonicus)[J].Marine Fisheries,2008,30(2). |
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| Authors: | YAN Wen-gang ZHANG Long-zhen ZHUANG Ping LIU Peng JIANG Qi |
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| Abstract: | Physiological characteristics and cryopreservation technology of Japanese croaker sperm were studied.The results showed that the pH of Japanese croaker sperm was 7.0-7.5,the density of sperm was(11.23±2.78)×109/ml.Sperm life-span was(229.33±17.16) s.Sperm motility and life-span were different under different salinities and pH.The activated sperm had the best motility(88.33±2.89)% under salinity of 30-35 and when pH was 7.5-8.5,the activated sperm had the highest motility(88±4.33)%.The sperm had longest life-span(229.33±17.16)s after being activated with salinity of 31.The lifespan of Japanese croaker sperm was 24 h under room temperature(25 ℃),whereas it was 36h under low temperature(4 ℃).The best diluter and cryoprotectant were choosen and fast cryopreservation of Japanese croaker sperm was also studied.The thawed sperm(38 ℃water) had highest motility(47±5.78)%under the D diluter with 20% ethyleneglycol as cryoprotectant. |
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| Keywords: | Japanese croaker sperm motility diluter cryoprotectants |
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