Effects of cryopreservation on sperm structure in Japanese pearl osyter <Emphasis Type="Italic">Pinctada fucata martensii</Emphasis> |
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| Authors: | Teruyoshi Narita Takayuki Kawamoto Kiyoshi Isowa Hideo Aoki Masahiro Hayashi Hiromi Ohta Akira Komaru |
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| Institution: | (1) Faculty of Bioresources, Mie University, 514-8507 Tsu, Mie, Japan;(2) Department of Fisheries, Graduate School of Agriculture, Kinki University, 631-8505 Nara, Japan;(3) Mie Prefectural Fish Farming Center, Hamajima, 517-0404 Shima, Mie, Japan;(4) Fisheries Research Division, Mie Prefectural Science and Technology Promotion Center, 517-0404 Shima, Mie, Japan |
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| Abstract: | To clarify factors reducing the motility and fertility of cryopreserved spermatozoa of the Japanese pearl oyster Pinctada fucata martensii, the structure of spermatozoa before and after cryopreservation was observed by scanning electron microscopy. Testicular
spermatozoa were diluted with cryopreservation diluent (10% methanol+18% fetal bovine serum+72% sea water), and dispensed
into 0.25-mL straws. The straws were cooled at a rate of approximately −20 °C/min to −50°C, and subsequently immersed in liquid
nitrogen. Percentage motility of spermatozoa before cryopreservation was 69.9±4.2%, and that of cryopreserved spermatozoa
was 24.0±1.8%, respectively. In cryopreserved spermatozoa, the percentage that lacked or had a deformed flagellum was 56.6±3.9%,
while in fresh spermatozoa this was 8.7±2.0%. In cryopreserved spermatozoa, the percentage of deformed acrosomes was 76.6±5.2%,
while in fresh spermatozoa this was only 0.9±0.3%. Cryopreserved spermatozoa with a normal acrosome and flagellum were only
15.4±3.5% of those in fresh spermatozoa. These results indicate that lesion of the flagellum and deformation of the acrosome
occurred through the cryopreservation procedure, and both types of damage lead to loss of the motility and fertility in thawed
spermatozoa. |
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| Keywords: | acrosome flagellum pearl oyster spermatozoa structure |
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