| 黄姑鱼异质雌核发育子代的遗传分析 |
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| 引用本文： | 谢仰杰,杨育凯,王志勇,蔡明夷,翁朝红.黄姑鱼异质雌核发育子代的遗传分析[J].中国水产科学,2020,27(11):1285-1294. |
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| 作者姓名： | 谢仰杰 杨育凯 王志勇 蔡明夷 翁朝红 |
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| 作者单位： | 集美大学水产学院, 农业农村部东海海水健康养殖重点实验室, 福建 厦门 361021;中国水产科学研究院南海水产研究所, 农业农村部南海渔业资源开发利用重点实验室, 广东 广州 510300;集美大学水产学院, 农业农村部东海海水健康养殖重点实验室, 福建 厦门 361021;青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266200 |
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| 基金项目： | 福建省高校产学合作项目（2018N5010；2014N5011）. |
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| 摘 要： | 为了解黄姑鱼（Nibea albiflora）异质雌核发育子代的基因纯合情况，利用微卫星标记（SSR）和扩增片段长度多态性标记（AFLP）对黄姑鱼异质雌核发育家系进行遗传鉴定和分析。结果显示：（1）雌核发育家系在4个SSR位点和5对AFLP引物组合扩增出的位点均未发现父本特异性等位条带，表明雌核发育体比率为100%。（2）用于遗传分析的7个SSR位点在雌核发育家系和正常交配家系中均未见完全纯合的情况，雌核发育家系7个SSR位点的平均纯合度为0.382，是正常交配家系（0.161）的2.37倍。雌核发育家系各个体的纯合位点数为0~6个，纯合位点所占比例为0~85.7%。（3）5对AFLP引物共扩增出182条清晰的扩增条带，其中有21条父本特异性条带和16条母本特异性条带。16条母本特异性条带中有7个条带在雌核发育家系中显著偏分离（P<0.05）。雌核发育家系和正常交配家系多态性条带比例分别为14.7%和20.3%。（4）雌核发育家系与母本的遗传相似度高于与正常交配家系的遗传相似度，正常交配家系同父本和母本的遗传距离大致相同。研究结果表明，黄姑鱼异质雌核发育二倍体家系的遗传纯合度显著高于正常交配家系，人工诱导雌核发育是促进基因纯合的一个有效途径，它不仅可以加速有利基因的纯合固定，还可以加速有害基因的淘汰，从而有效提高育种效率。
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| 关 键 词： | 黄姑鱼 雌核发育 遗传分析 基因纯合度 |
| 收稿时间： | 2020/6/23 0:00:00 |
| 修稿时间： | 2020/7/21 0:00:00 |
Genetic analysis of yellow drum Nibea albiflora meiotic gynogens |
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| XIE Yangjie,YANG Yukai,WANG Zhiyong,CAI Mingyi,WENG Zhaohong.Genetic analysis of yellow drum Nibea albiflora meiotic gynogens[J].Journal of Fishery Sciences of China,2020,27(11):1285-1294. |
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| Authors: | XIE Yangjie YANG Yukai WANG Zhiyong CAI Mingyi WENG Zhaohong |
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| Institution: | Fisheries College, Jimei University, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Xiamen 361021, China;Key Laboratory of South China Sea Fishery Research Exploitation & Utilization, Ministry of Agriculture and Rural Affairs;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;Fisheries College, Jimei University, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Xiamen 361021, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China |
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| Abstract: | The yellow drum (Nibea albiflora) is an important marine fish and has been cultured widely in Southeast China in recent years. To understand the gene homozygosity of meiotic gynogens of yellow drum, microsatellite markers (SSR) and amplified fragments length polymorphism (AFLP) markers were applied to identify gynogenetic diploid offspring and genetic analyze. The results showed:(1) no paternal specific alleles were found in all 4 SSR loci or amplification loci from five pairs of AFLP selective primers in the gynogenetic diploid family, indicating that all individuals in the gynogenetic diploid family were gynogens; (2) no completely homozygous loci were found in seven SSR loci in either gynogenetic diploid family or normal mating family. The average homozygosity of the gynogenetic diploid family was 0.382, 2.37 times that of the normal mating family (0.161). Homozygous loci in gynogenesis individuals was during 0-6, with their homozygous ratio of 0-85.7%, whereas the homozygous loci in normal individuals was during 0-4, with their homozygous ratio of 0-57.1%; (3) 182 clear bands were amplified from five pairs of AFLP selective primers, of which there were 21 paternal and 16 maternal specific bands. Among the 16 maternal specific bands, ten bands were heterozygous loci, and seven loci in the gynogenetic diploid family showed significant partial separation (P<0.05). The percentage of polymorphic bands in the gynogenetic and normal mating families were 14.7% and 20.3%, respectively; (4) based on SSR and AFLP markers, the gynogenetic diploid family had a higher genetic similarity to female parents than to the normal mating family, whereas the normal mating family had approximately the same genetic distance to both parents. The results showed that the genetic homozygosity of the meiotic gynogenetic diploid family was significantly higher than that of the normal mating family, and artificial induction of gynogenetic development is an effective way to promote gene homozygosity, since it can accelerate favorable homozygous gene fixation, and also accelerate harmful gene elimination; thus, effectively improve breeding efficiency. |
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| Keywords: | Nibea albiflora yellow drum genetic analysis gene homozygosity |
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