| 两种颜色栉孔扇贝卵及其早期胚胎磷脂和核酸含量的比较 |
| |
| 引用本文： | 周丽青,杨爱国,刘志鸿,王清印.两种颜色栉孔扇贝卵及其早期胚胎磷脂和核酸含量的比较[J].中国水产科学,2006,13(5):745-748. |
| |
| 作者姓名： | 周丽青 杨爱国 刘志鸿 王清印 |
| |
| 作者单位： | 中国水产科学研究院,黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东,青岛,266071 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划);国家科技攻关项目 |
| |
| 摘 要： | 用钼蓝比色法和紫外分光光度法分别测定栉孔扇贝（Chlamys farrPri）两种颜色的卵子及其早期胚胎中磷脂含量和核酸含量，并进行比较。结果表明，橘红色卵子的RNA含量较浅黄色的略高，而两种颜色卵子的DNA含量却非常相似；在2细胞期，高受精率的受精卵其RNA量迅速增加，DNA和磷脂含量变化不大，但低受精率的受精卵相对未受精卵而言，其DNA、RNA和磷脂含量略有降低；在32～64细胞期，受精率高的胚胎中DNA和磷脂含量增加幅度大，RNA基本保持恒定。磷脂含量与卵子或胚胎的颜色可能有关，核酸、磷脂含量的变化与受精率高低有关。本实验旨为鉴定扇贝卵子的质量提供了科学依据。
|
| 关 键 词： | 栉孔扇贝 卵 早期胚胎 磷脂 核酸 |
| 文章编号： | 1005-8737-（2006）05-0745-04 |
| 收稿时间： | 2005-09-16 |
| 修稿时间： | 2005-12-30 |
Comparison of phospholipid and nucleic acid content in scallop Chlamys farreri eggs and their early embryos with two colors |
| |
| ZHOU Li-qing,YANG Ai-guo,LIU Zhi-hong,WANG Qing-yin.Comparison of phospholipid and nucleic acid content in scallop Chlamys farreri eggs and their early embryos with two colors[J].Journal of Fishery Sciences of China,2006,13(5):745-748. |
| |
| Authors: | ZHOU Li-qing YANG Ai-guo LIU Zhi-hong WANG Qing-yin |
| |
| Institution: | Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research h stitute , Chinese Academy of Fishery Sciences, Qingdao 266071, China |
| |
| Abstract: | The content change of phospholipid and nucleic acid in scallop Chlamys farreri eggs with two different colors and their early embryos were mensurated by molybdenum blue spectrophotometry and ultraviolet spectrophotometry respectively, and were compared at the same time. The result showed that the content of RNA in orange eggs was higher than that in yellow eggs; while the content of DNA in two different colors eggs was quite similar;eggs began division after fertilization, at two-celled stage,the RNA content of zygotes with higher fertilization rate grew faster, while the DNA and phospholipid content only varied a little, in those zygotes with lower developing rate, the DNA,RNA and phospholipid content even reduced a little;at 32 or 64-cell stage, the content of DNA and phospholipid in embryos with higher fertilization rate grew faster, while the RNA content kept constant. Though there is only some relation between the content of phospholipid and the colors of eggs and embryos, the content change of nucleic acid and phospholipid is connected with the fertilization rate. The result of this experiment could offer reliable data for identifying eggs quality. |
| |
| Keywords: | Chlamys farreri eggs early embryos phospholipid nucleic acid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
