| 基于线粒体COⅠ基因和形态学方法的3种梭子蟹科溞状幼体物种鉴定 |
| |
| 引用本文： | 张新贵,何天庆,叶振江,王艺翰,张文超,熊文清,曲宁新,蒋慧东,卢志光.基于线粒体COⅠ基因和形态学方法的3种梭子蟹科溞状幼体物种鉴定[J].中国水产科学,2023,30(2):218-225. |
| |
| 作者姓名： | 张新贵 何天庆 叶振江 王艺翰 张文超 熊文清 曲宁新 蒋慧东 卢志光 |
| |
| 作者单位： | 中国海洋大学水产学院, 山东 青岛 266003;日照山海天旅游度假区海洋发展局, 山东 日照 276827 |
| |
| 基金项目： | 国家重点研发计划项目(2018YFD0900903)； 国家自然科学基金项目(41876177). |
| |
| 摘 要： | 为调查黄海蟹类资源情况,并识别梭子蟹科溞状幼体各阶段形态,利用2018年5月下旬至8月上旬在黄海海州湾及邻近水域的鱼类浮游生物大面综合调查获得的海洋蟹类幼体样品,基于线粒体COⅠ基因和形态学方法对梭子蟹科溞状幼体种类进行了鉴定。结果显示,春夏季调查水域共出现3种梭子蟹科幼体:三疣梭子蟹(Portunus trituberculatus)、日本蟳(Charybdis japonica)和双斑蟳(Charybdis bimaculata);线粒体COⅠ基因测序可准确识别3个物种。对3种梭子蟹溞状幼体的形态学观察结果表明,背棘与额棘形态、尾叉外缘小刺的有无可作为区分上述3种梭子蟹溞状幼体的主要形态学指标。根据3个物种幼体形态特征建立了梭子蟹科溞状幼体分类检索表,基于检索表对幼体进行分类鉴定,并选择8月上旬航次形态学鉴定的80个三疣梭子蟹溞状幼体、24个日本蟳溞状幼体、70个双斑蟳溞状幼体样品进行分子测序验证。结果表明,上述3种蟹类溞状幼体的形态学鉴定准确率分别达到93.75%、100%和97.14%,误判皆发生于溞状幼体Ⅰ期梭子蟹科3个物种之间。研究初步建立了黄海中部梭子蟹科溞状幼体形态鉴定...
|
| 关 键 词： | 黄海 梭子蟹科 溞状幼体 鉴定 形态 COⅠ基因 |
Zoea identification of three swimming crabs: Portunus trituberculatus, Charybdis japonica, and Charybdis bimaculate through morphological and mitochondrial CO I gene analyses |
| |
| ZHANG Xingui,HE Tianqing,YE Zhenjiang,WANG Yihan,ZHANG Wenchao,XIONG Wenqing,QU Ningxin,JIANG Huidong,LU Zhiguang.Zoea identification of three swimming crabs: Portunus trituberculatus, Charybdis japonica, and Charybdis bimaculate through morphological and mitochondrial CO I gene analyses[J].Journal of Fishery Sciences of China,2023,30(2):218-225. |
| |
| Authors: | ZHANG Xingui HE Tianqing YE Zhenjiang WANG Yihan ZHANG Wenchao XIONG Wenqing QU Ningxin JIANG Huidong LU Zhiguang |
| |
| Institution: | College of Fisheries, Ocean University of China, Qingdao 266003 , China; Rizhao Shanhaitian Tourism Resort Marine Development Bureau, Rizhao 276827 , China |
| |
| Abstract: | Swimming marine crabs are an important fishing resource worldwide. Early life history is a key process for crab stock biomass variation and has great research necessity and importance for scientific management and protection of fisheries resources. Standards for zoea morphological identification are an important key base for studying crab early life history, and some studies have described the zoeal stages of swimming crabs from the Yellow Sea in recent decades. However, no studies have been conducted on key forms of identification of crab larvae in this area. Based on the ichthyoplankton field survey conducted along the Haizhou Bay and adjacent waters in the Yellow Sea from May to August in 2018, we explored the larvae identification method of three species of marine swimming crabs (Crustacea: Portunidae) through morphological and mitochondrial CO I gene analyses. More than 150 crab larvae were randomly selected, observed for morphological characteristics, and identified by mitochondrial CO I gene analysis. Altogether, three Portunidae species, Portunus trituberculatus, Charybdis japonica, and Charybdis bimaculate, were found among them, and the results indicated that dorsal spine morphology and the number of armatures of lateral telson forks could be used for key classification characteristics for these three crab zoeae. As a result, a key for the identification of zoeal stages of three Portunidae crabs was provided. Additionally, 80 individuals of Portunus trituberculatus zoeae (20 ind. for each stage), 24 individuals of Portunus trituberculatus zoeae (four ind. for each stage), and 70 individuals of Portunus trituberculatus zoeae (10 ind. for each stage) from the August cruise were morphologically identified and examined using the mitochondrial CO I gene analysis; the morphological identification accuracies were 93.75%, 100%, and 97.14% for these three species, respectively. The study established a morphological identification standard for zoeae of the three Portunidae crabs and provided a base for their early life history research. The biodiversity samples for molecular sequencing analysis were collected from May to August, and no other Portunidae larvae species were found. Therefore, the morphological identification standard of Portunidae larvae could be employed in research of crab early life history in spring and summer in the coastal Yellow Sea. However, different Portunidae crabs may exist in other marine waters or seasons, and further crab larval morphological research should be done in the future. Meanwhile, 14 Portunidae megalops were identified based on mitochondrial CO I gene analysis in this study, but no systemic morphology study was conducted for them because of the lack of samples, which may be related to their nocturnal diel vertical migration behavior and the surface tows sampling method in day time. The results help to promote the research of crab stock variation and the sustainable utilization of fisheries resources, and has important implications for exploring the early life history of crabs in this region. |
| |
| Keywords: | |
|
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
|
