| 基于S7核糖体蛋白基因部分序列的7种金线鱼属鱼类分子系统进化关系 |
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| 引用本文： | 梁日深,王超,邹青,周爱国,邹记兴.基于S7核糖体蛋白基因部分序列的7种金线鱼属鱼类分子系统进化关系[J].中国水产科学,2013,20(3):506-513. |
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| 作者姓名： | 梁日深 王超 邹青 周爱国 邹记兴 |
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| 作者单位： | 1. 华南农业大学 动物科学学院, 广东 广州 510642
2. 清远市北江水产科学研究所, 广东 清远 511500 |
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| 基金项目： | 国家自然科学基金项目(30972258, 30771652). |
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| 摘 要： | 测定了7种金线鱼属(Nemipterus)及2种锥齿鲷属(Pentapus)鱼类S7核糖体蛋白基因(S7基因)内含子1部分序列,以二线眶棘鲈(Scolopsis bilineatus)做为外类群初步探讨了其分子进化关系.测序所得S7部分序列为734~746 bp,序列比对后得到同源序列743 bp.其中保守位点386个,变异位点351个,简约性信息位点289个.A+T含量(54.1%)高于G+C含量(45.9%).基于Kimura 2-Parameter模型计算出7种金线鱼的遗传距离为0.042~0.294.S7序列存在大量碱基插入与缺失,其中日本金线鱼(Nemipterus japonicus)与苏门答腊金线鱼(N. mesoprion)在第167、182、474、608、662 bp位置,金线鱼(N. virgatus)与印度洋金线鱼(N. bipunctatus)第227、332、401、604 bp的位置具有相同的碱基插入缺失特征,且具有种类特异性.最后利用最大似然法(ML)与贝叶斯分析(BI)构建分子系统进化树,7种金线鱼聚在一起,其中日本金线鱼与苏门答腊金线鱼聚为一支,金线鱼、深水金线鱼和印度洋金线鱼聚成一支.结论认为需要结合多方面的形态与分子证据,才能进一步明确金线鱼属鱼类的系统进化关系.
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| 关 键 词： | 金线鱼属 系统发育 S7核糖体蛋白基因 |
| 修稿时间： | 2013/5/28 0:00:00 |
Molecular phylogenetic relationship among seven Nemipterus species based on partial S7 ribosomal protein gene sequences |
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| LIANG Rishen,WANG Chao,YAO Donglin,ZOU Qing,ZHOU Aiguo,ZOU Jixing.Molecular phylogenetic relationship among seven Nemipterus species based on partial S7 ribosomal protein gene sequences[J].Journal of Fishery Sciences of China,2013,20(3):506-513. |
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| Authors: | LIANG Rishen WANG Chao YAO Donglin ZOU Qing ZHOU Aiguo ZOU Jixing |
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| Institution: | 1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China2. Qingyjuan North River Fishery Science Institute, Qingyuan 511500, China |
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| Abstract: | species, belonging to the family Nemipteridae, order Perciformes, are widely distributed in the tropical and subtropical waters of the Indo-Western Pacific. Morphologically, a number of closely related species share similar external coloration and patterns, resulting in difficulty identifying and classifying them based on morphological evidence alone. Despite this, priorevaluation of has primarily relied on morphological data, with few molecular phylogenetic data currently available for this genus. We analyzed the molecular phylogenetic relationship among seven common species collected from the Indo-Western Pacific. Partial sequences of the 1st intron of the S7 ribosomal protein gene were obtained for seven species. We used sequence data from sequences ranging from 734 bp to 746 bp and homologous sequences of 743 bp were obtained for phylogenetic analysis after alignment. Of the 743 bp aligned sequences, 386 sites were conservative, 351 sites were variable, and 289 were parsimony-informative. The content of A+T (54.1%)was lower than that of G+C (45.9%). The genetic distance among the seven , calculated using a Kimura two-arameter model, were between 0.042 and 0.294. We observed a number of insertions and deletions in the S7 ribosomal protein gene sequences. The insertions and deletions at 167 bp, 182 bp, 474 bp, 608 bp, 662 bp within , and at 227 bp, 332 bp, 401 bp, and 604 bp within were identical. We constructed molecular phylogenetic trees using the maximum likelihood and Bayesian inference methods. The seven N. japonicus and N. bipunctatus and were clustered in a separate clade. These phylogenetic relationships were consistent with their morphological classifications. However, the phylogenetic position of Nemipterus species were divided into two morphologically distinct groups according to the length of upper and lower caudal fin lobes. The two lobes are of equal length in have an elongated trailing filament from their upper caudal fin and the upper fin lobe is longer than the lower. Our results suggest acombination of more morphological and molecular evidence is needed for further phylogenetic studies of the genus. |
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| Keywords: | Nemipterus phylogeny S7 ribosomal protein gene |
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