| 鲤血清转铁蛋白纯化及其与铁离子的结合特性 |
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| 引用本文： | 龙华,曾勇,刘曼西.鲤血清转铁蛋白纯化及其与铁离子的结合特性[J].中国水产科学,2000,7(1):25-30. |
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| 作者姓名： | 龙华 曾勇 刘曼西 |
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| 作者单位： | 1. 中国水产科学研究院,长江水产研究所,荆州,434000
2. 华中理工大学,生物系,湖北,430074 |
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| 基金项目： | 农业部“948”资助项目! (9830 94) |
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| 摘 要： | 用硫酸铵分级沉淀法及制备性ＳＤＳ－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）法，获得含有鲤（Ｃｙｐｒｉｎｕｓｃａｒｐｉｏ）血清转铁蛋白（Ｔｆ）的ＰＡＧ条带，同时除去ＳＤＳ及考马斯亮蓝，分离纯化了转铁蛋白，并做铁离子结合试验及光谱分析。结果表明，转铁蛋白结合铁离子后的特异吸收峰为４６７ｎｍ，说明，去ＳＤＳ后转铁蛋白仍有很强的铁离子结合能力，用普通ＳＤＳ－ＰＡＧＥ法测得鲤血清Ｔｆ分子量约为７４０００Ｄａｌ
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| 关 键 词： | 鲤 血清 转铁蛋白 纯化 铁离子 |
| 修稿时间： | 1999-04-16 |
Purification of transferrin in Cyprinus carpio serum and its iron-binding properties |
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| LONG Hua,ZENG Yong,LIU Man-xi.Purification of transferrin in Cyprinus carpio serum and its iron-binding properties[J].Journal of Fishery Sciences of China,2000,7(1):25-30. |
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| Authors: | LONG Hua ZENG Yong LIU Man-xi |
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| Abstract: | With the methods of ammonium sulfate grade subsidence and producing sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), the stripped polyacrylamide gel(PAG) of transferrin in C. carpio serum was obtained. Then SDS and Coomassie blue were eliminated from the serum and the transferrin in C. carpio serum was purified and the ferric iron binding test was performed. The SDS-PAGE spectrums of grade subsicence components and puified sample of C. carpio serum were analysed. The results show that the special absorbing peak of transferrin is at 467 nm for iron binding; the transferin still has a stronger capability to bind iron ions after SDS was eliminated; the molecular weight of C. carpio serum transferrin is about 74 000 Dalton tested with normal SDS-PAGE method. |
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| Keywords: | Cyprinus carpio serum transferrin purification ferric iron |
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