Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthopterygian fishes |
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| Authors: | Email author" target="_blank">A?ShimizuEmail author T?Sakai K?Nashida H?Honda |
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| Institution: | (1) National Research Institute of Fisheries Science, Fisheries Research Agency, Fukuura 2-12-4, Kanazawa Yokohama, 236-8648, Japan;(2) Department of Animal and Marine Bioresource, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan;(3) Kochi Station, National Research Institute of Fisheries Science, Fisheries Research Agency, Sanbashidori 6-1-21, Kochi 780-8010, Japan;(4) Present address: Research Promotion and Development Department, Fisheries Research Agency, Queen s Tower B, Nishi-ku Yokohama, 220-6115, Japan |
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| Abstract: | Pituitaries of various teleosts belonging to 25 orders were immunostained with antisera raised against synthetic fragment peptides corresponding to conservative regions of gonadotropin subunits (mummichog FSH 50-60 and mummichog LH 91-106). Both immunoreactive FSH cells and immunoreactive LH cells were successfully identified in the fishes of almost every order of the superorder Acanthopterygii and several species of the superorders Paracanthopterygii and Polymixiomorpha, such as mullet, alfonsino, flyingfish, mackerel, flounder, cod, beardfish, etc. These antisera are therefore considered as universal antisera for immunocytochemical application to acanthopterygian fishes. Extensive diversity in the abundance of the FSH cells and the LH cells among species was noted even in fishes with similar gonadal stages, indicating the possibility that the respective roles of FSH and LH may vary considerably among species in advanced teleosts.Evident but generally weak immunoreactivities to anti-mummichog LH 91-106 were observed in the fishes of the superorder Cyclosquamata; and slight or weak immunoreactivities to the antiserum were observed in the fishes of several more primitive taxa (superorder Stenopterygii, Protacanthopterygii, Ostariophysi, subdivision Clupeomorpha, and subdivision Elopomorpha). No immunoreactivity to anti-mummichog FSH 50-60 was observed in these fishes. These results are consistent with the phylogenetic status of the fishes and the degree of conservativeness in the amino acid sequences of the antigen regions. |
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| Keywords: | Acanthopterygii Follicle stimulating hormone (FSH) gonadotroph immunocytochemistry Luteinizing hormone (LH) phylogenetic status pituitary synthetic peptide teleost universal antiserum |
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