Isolation,Identification and Drug Resistance Analysis of Pathogenic Enterococcus feacium of Sheep |
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| Authors: | LIU Yong-an LI Xue-rui ZHOU Jian-hua MA Li-na LIU Yong-sheng |
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| Institution: | Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China |
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| Abstract: | To further identify the pathogenic strains and analyze their antibiotic resistance, the methods of pathogen isolating, the methods of Gram staining, biochemical tests and 16S rRNA PCR amplification, virulence tests, drug sensitive tests, virulence genes and resistance genes PCR amplification were used. The results showed that the strain was Gram-positive and revealed positive after reacting with 6.5% NaCl, melibiose, sucrose and etc., while revealed negative reactions with VP, hippurate, arabinose and etc.. It showed 100% similarity with Enterococcus feacium 16S rRNA gene sequence in GenBank after PCR amplification of 16S rRNA gene and BLAST alignment. It was found severe pathogenicity after virulence tests in mice. The strain was highly resistant to oxacillin, penicillin G of β-lactam antibiotics and norfloxacin, ciprofloxacin, levofloxacin of quinolones antibiotics and tetracycline, while was sensitive to antibiotics of erythromycin, vancomycin and clindamycin. It revealed that virulence factor genes Asal, cylA, acm and resistance genes TetM, ant(6)-Ⅰ, aac(6')-aph (2"), ermB showed positive. The results showed that the bacteria was Enterococcus feacium, it had a strong pathogenicity and severe drug resistance, which might be related to the highly positive rates of virulence genes and drug resistance genes. |
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| Keywords: | Enterococcus feacium isolation and identification drug resistance virulence factor resistance gene |
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