| 超高效液相色谱—串联质谱法测定牛奶中6种玉米赤霉烯酮类霉菌毒素的残留 |
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| 引用本文： | 韩镌竹,田晓玲,丛鑫,李香珍,苏崴艺,李永才,李欣南.超高效液相色谱—串联质谱法测定牛奶中6种玉米赤霉烯酮类霉菌毒素的残留[J].中国畜牧兽医,2013,40(Z1):46-49. |
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| 作者姓名： | 韩镌竹 田晓玲 丛鑫 李香珍 苏崴艺 李永才 李欣南 |
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| 作者单位： | 1. 辽宁省兽药饲料畜产品质量安全检测中心, 辽宁沈阳 110016;2. 盘锦市食品药品检验所, 辽宁盘锦 124010 |
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| 摘 要： | 试验旨在建立牛奶中6种玉米赤霉烯酮类霉菌毒素残留的超高效液相―串联质谱检测方法。牛奶样品经乙腈提取，免疫亲和柱净化，C18色谱柱分离，以0.02%（V/V）乙酸―水/甲醇为流动相，采用梯度洗脱方式进行液相色谱分离，多反应监测模式，外标法进行定量。牛奶中6种玉米赤霉烯酮类霉菌毒素的检测限可达到1.0 μg/L，且在2.5～100.0 μg/L的浓度范围内，线性关系良好。该方法专属性强、灵敏度高、分离度好，适用于牛奶中的6种玉米赤霉烯酮类霉菌毒素残留的测定。
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| 关 键 词： | 玉米赤霉烯酮类 超高效液相色谱―串联质谱 牛奶 |
| 收稿时间： | 2013-08-01 |
Determination on 6 Kinds of Corn Gibberellic Olefine Ketone Residues in the Cow Milk by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| HAN Juan-zhu,TIAN Xiao-ling,CONG Xin,LI Xiang-zhen,SU Wei-yi,LI Yong-cai,LI Xin-nan.Determination on 6 Kinds of Corn Gibberellic Olefine Ketone Residues in the Cow Milk by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J].China Animal Husbandry & Veterinary Medicine,2013,40(Z1):46-49. |
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| Authors: | HAN Juan-zhu TIAN Xiao-ling CONG Xin LI Xiang-zhen SU Wei-yi LI Yong-cai LI Xin-nan |
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| Institution: | 1. Liaoning Quality Inspection and Test Center of Veterinary Feed and Animal Products, Shenyang 110016, China;2. Panjin Institute for Food and Grug Control, Panjin 124010, China |
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| Abstract: | Developing a method for determination on 6 kinds of corn gibberellic olefine ketone (α-zearalanol, β-zearalanol, α-zearalenol, β-zearalenol, zearalanone and zearalenone) in the milk. The milk was concentrated after extracted with acetonitrile and purified by immunoaffinity solid-phase extraction and then was determined on an ACQUITY UPLCTM BEH C18 column with gradient elution using methanol and water (containing 0.02% acetic acid) as mobile phases. The mass spectrometric acquisitions were carried out by means of multiple reaction monitoring (MRM) in electrospray negative ionization mode. The detection limits were 1.0 μg/L for 6 kinds of corn gibberellic olefine ketone. The calibration curves were linear from 2.5～100.0 μg/L. This method was specific, sensitive, and suitable for determination on 6 kinds of corn gibberellic olefine ketone in the milk. |
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| Keywords: | corn gibberellic olefine ketone ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) cow milk |
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