| 超高效液相色谱—串联质谱法测定生鲜乳中6种农药的残留 |
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| 引用本文： | 韩镌竹,田晓玲,李香珍,李欣南,苏崴艺,李永才,丛鑫.超高效液相色谱—串联质谱法测定生鲜乳中6种农药的残留[J].中国畜牧兽医,2013,40(Z1):62-67. |
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| 作者姓名： | 韩镌竹 田晓玲 李香珍 李欣南 苏崴艺 李永才 丛鑫 |
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| 作者单位： | 1. 辽宁省兽药饲料畜产品质量安全检测中心, 辽宁沈阳 110016;2. 盘锦市食品药品检验所, 辽宁盘锦 124010 |
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| 摘 要： | 本试验建立了生鲜乳中西草净、枯草隆、西码津、莠去津、乙草胺和丁草胺残留量的超高效液相―串联质谱检测方法。牛奶样品用乙腈提取，经固相萃取柱净化，C18色谱柱分离，以0.1%（V/V）甲酸―水/乙腈为流动相，采用梯度洗脱方式进行液相色谱分离，多反应监测模式，外标法进行定量。结果发现，西草净、枯草隆、西码津、莠去津、乙草胺和丁草胺的检测限分别为0.1、1.0、0.2、0.1、4.0和4.0 μg/L，线性范围分别为1.0～50.0、10.0～500.0、1.0～50.0、1.0～50.0、20.0～1000.0和20.0～1000.0 μg/L。表明该方法专属性强、灵敏度高、分离度好，适用于生鲜乳中西草净、枯草隆、西码津、莠去津、乙草胺和丁草胺残留量的测定。
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| 关 键 词： | 超高效液相色谱―串联质谱 农药 生鲜乳 |
| 收稿时间： | 2013-08-01 |
Determination on 6 Kinds of Pesticide Residues in the Raw Milk by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| HAN Juan-zhu,TIAN Xiao-ling,LI Xiang-zhen,LI Xin-nan,SU Wei-yi,LI Yong-cai,CONG Xin.Determination on 6 Kinds of Pesticide Residues in the Raw Milk by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J].China Animal Husbandry & Veterinary Medicine,2013,40(Z1):62-67. |
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| Authors: | HAN Juan-zhu TIAN Xiao-ling LI Xiang-zhen LI Xin-nan SU Wei-yi LI Yong-cai CONG Xin |
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| Institution: | 1. Liaoning Quality Inspection and Test Center of Veterinary Feed and Animal Products, Shenyang 110016, China;2. Panjin Institute for Food and Grug Control, Panjin 124010, China |
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| Abstract: | The author established a kind of method to check the residues of simetryn,chloroxuron,simazine,atrazine,acetochlor,butachlor in milk. The samples were extracted by acetonitrile,purified by solid phase extraction,and were determined by an ACQUITY UPLCTM BEH C18 column with gradient elution using acetonitrile and water (containing 0.1% formic acid) as mobile phase. The mass spectrometric acquisitions were carried out by means of multiple reaction monitoring (MRM) in electrospray positive ionization mode. The detection limits of simetryn,chloroxuron,simazine,atrazine,acetochlor,butachlor were 0.1,1.0,0.2,0.1,4.0 and 4.0 μg/L. The calibration curves were liner from 1.0～50.0,10.0～500.0,1.0～50.0,1.0～50.0,20.0～1000.0 and 20.0～1000.0 μg/L.The results showed that this method had specific, sensitive,and suitable for determination on simetryn,chloroxuron,simazine,atrazine,acetochlor,butachlor in the milk. |
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| Keywords: | ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) pesticide milk |
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